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US 2010/0143457 A1Published application
Extended soluble PH20 polypeptides and uses thereof
AssigneeHalozyme, Inc.
InventorsGe Wei, Krishnasamy Panneer Selvam, Louis Bookbinder, Gregory I. Frost
Life of this patent
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Abstract
Soluble PH20 polypeptides are provided, including extended soluble PH20 polypeptides, and uses thereof. Also provided are other C-terminally truncated PH20 polypeptides and partially deglycosylated PH20 polypeptides and uses thereof.
Claims89
An extended soluble PH20 hyaluronidase (esPH20).
A truncated PH20 hyaluronidase selected from among:
- a) a truncated PH20 hyaluronidase that consists of the sequence of amino acids set forth as amino acids 1-465, 1-491, 1-492, 1-493, 1-494, 1-495, 1-496, 1-497, 1-498, 1-499 or 1-500 of SEQ ID NO:
- 107;
- b) a truncated PH20 hyaluronidase that contains amino acid substitutions in the sequence of amino acids set forth as amino acids 1-465, 1-491, 1-492, 1-493, 1-494, 1-495, 1-496, 1-497, 1-498, 1-499 or 1-500 of SEQ ID NO:
- 107, whereby the amino acid-substituted PH20 hyaluronidase consists of a sequence of amino acids that has at least 85%, 87%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity with the sequence of amino acids set forth as amino acids 1-465, 1-491, 1-492, 1-493, 1-494, 1-495, 1-496, 1-497, 1-498, 1-499 or 1-500 of SEQ ID NO:
- 107;
- c) a truncated PH20 hyaluronidase that consists of the sequence of amino acids set forth as amino acids 36-465, 36-484, 36-485, 36-486, 36-487, 36-488 or 36-489 of SEQ ID NO:
- 107;
- d) a truncated PH20 hyaluronidase that contains amino acid substitutions in the sequence of amino acids set forth as amino acids 36-465, 36-484, 36-485, 36-486, 36-487, 36-488 or 36-489 of SEQ ID NO:
- 107, whereby the amino acid-substituted PH20 hyaluronidase consists of a sequence of amino acids that has at least 85%, 87%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity with the sequence of amino acids set forth as amino acids 36-465, 36-484, 36-485, 36-486, 36-487, 36-488 or 36-489 of SEQ ID NO:
- 107;
- e) a truncated PH20 hyaluronidase that is a glycoprotein that consists of the sequence of amino acids set forth in any of SEQ ID NOS:
- 55-106, wherein the PH20 hyaluronidase glycoprotein is N-glycosylated but is not glycosylated at one or more amino acid residues corresponding to amino acid residues 82, 166 or 254 of SEQ ID NO:
- 107;
- or
- f) a truncated PH20 hyaluronidase that is a glycoprotein that contains amino acid substitutions in the sequence of amino acids set forth in any of SEQ ID NOS:
- 55-106, whereby the amino acid-substituted PH20 hyaluronidase consists of a sequence of amino acids that has at least 85%, 87%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity with the sequence of amino acids set forth in any of SEQ ID NOS:
- 55-106, wherein the PH20 hyaluronidase glycoprotein is N-glycosylated but is not glycosylated at one or more amino acid residues corresponding to amino acid residues 82, 166 or 254 of SEQ ID NO:
- 107.
15 more elements
A PH20 hyaluronidase glycoprotein, comprising the sequence of amino acids set forth in SEQ ID NO:
- 107, or a truncated neutral active form thereof, or a sequence of amino acids that has at least 85%, 87%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity with the sequence of amino acids set forth in SEQ ID NO:
- 107 or truncated forms thereof, wherein the PH20 hyaluronidase glycoprotein is N-glycosylated but is not glycosylated at one or more amino acid residues corresponding to amino acid residues 82, 166 or 254 of SEQ ID NO:
- 107.
A nucleic acid molecule that is an expression vector, comprising a polynucleotide that consists of the sequence of nucleotides set forth in any of SEQ ID NOS:
- 150-154, 200-201 and 213-215, or degenerates thereof and includes a stop codon to produce a truncated PH20 or esPH20 encoded by any of SEQ ID NOS:
- 150-154, 200-201 and 213-215, wherein the polynucleotide is operatively linked for expression to produce the encoded truncated PH20 or esPH20.
Dependencies are inferred from the claim text.