Biological artificial ligament and method of making

A method of preparing a natural animal ligament or tendon for implantation into a human body, comprising the steps of:

• isolating from a host a natural animal ligament or tendon that has a substrate;
• crosslinking and fixing the substrate;
• blocking residual specific active groups in protein molecules of the substrate after fixation by applying at least one active reagent;
• altering the specific conformation of protein molecules of the substrate by a reagent with strong hydrogen bonding power;
• and
• coupling an active layer to the substrate that includes either a polypeptide or a glucosaminoglycan that has the ability to adhere growth factors after implantation.