Treatment of t-cell mediated diseases
AssigneeDmi Biosciences, Inc.
InventorsDavid Bar-Or, Raphael Bar-Or, Richard Shimonkevitz
Life of this patent
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Abstract
The invention provides a method of treating T-cell mediated diseases and a method of inhibiting the activation of T-cells using certain diketopiperazines. The invention also provides methods of synthesizing diketopiperazines and pharmaceutical compositions comprising certain diketopiperazines. The invention further provides methods of making improved pharmaceutical compositions of proteins and peptides by either increasing or decreasing the content of diketopiperazines in the compositions and the resultant improved pharmaceutical compositions.
Claims55
1-44. (canceled)
A method of treating a T-cell mediated disease comprising administering to an animal in need thereof an effective amount of a pharmaceutical composition comprising a protein or peptide normally found in the animal, the protein or peptide having been treated so that the composition also comprises at least one diketopiperazine derived from the protein or peptide.
A method of inhibiting T-cell activation comprising administering to an animal in need thereof an effective amount of a pharmaceutical composition comprising a protein or peptide normally found in the animal, the protein or peptide having been treated so that the composition also comprises at least one diketopiperazine derived from the protein or peptide.
A method of synthesizing a diketopiperazine comprising heating a solution of a protein or peptide under conditions effective to cause the formation of the diketopiperazine.
A method of synthesizing a diketopiperazine comprising contacting a solution of a protein or peptide with an enzyme that cleaves the two N-terminal or the two C-terminal amino acids of the protein or peptide under conditions effective to produce the diketopiperazine.
An improved pharmaceutical composition of a protein or peptide, the improvement comprising a decreased content of diketopiperazines in the composition.
A method of making an improved pharmaceutical composition of a protein or peptide, the method comprising removing from the composition at least some of the diketopiperazines present in the composition.
A method of making an improved pharmaceutical composition of a protein or peptide, the method comprising treating a solution of the protein or peptide so as to increase the content of diketopiperazines.
An improved pharmaceutical composition of a protein or peptide, the improvement comprising an increased content of diketopiperazines in the composition.
A pharmaceutical composition comprising a diketopiperazine in a filtrate from size-exclusion chromatography of a solution of a protein or peptide.
Dependencies are inferred from the claim text.