Use of bone marrow cells for long term culture of pancreatic islet cells
AssigneeRoger Williams Hospital
InventorLuguang Luo
Life of this patent
Estimating the term…
Abstract
Bone marrow cells have been demonstrated to be useful in the maintenance of pancreatic islet β cell viability, structure, and/or function in culture for a sustained period. Bone marrow cells were also found to promote β cell growth while reducing inflammatory cytokine release and reduce apoptosis. Moreover, islet cells co-cultured with bone marrow cells were shown to retain insulin response function and to function in an islet cell transplant in a mouse model of diabetes to restore normal insulin secretion. Cord blood cells and isolated peripheral CD34+ blood cells were unable to support β islet cell growth or increase survival.
Claims23
A method for culturing islet β cell comprising culturing an islet β cell with a bone marrow cell in vitro.
4-6. (canceled)
(canceled)
A method for pancreatic islet β cell expansion in vitro comprising, culturing an islet β cell with a bone marrow cell.
A method for increasing islet β cell viability, improved islet β cell function, and/or improved β cell morphology comprising culturing an islet β cell with a bone marrow cell in vitro.
18-24. (canceled)
A method of treating a subject in need of an islet β cell transplant comprising culturing an islet β cell with a bone marrow cell in vitro;
- and implanting the islet cell and the bone marrow cell into the individual in need of an islet β cell transplant.
26-30. (canceled)
A method of treating diabetes in a subject in need thereof comprising:
- culturing an islet β cell with a bone marrow cell in vitro;
- and
- implanting the cultured islet β cell into the subject;
- thereby treating the subject for diabetes.
32-36. (canceled)
A method for implanting islet β cells in a subject comprising:
- culturing an islet β cell with a bone marrow cell in vitro;
- and
- implanting the cultured islet β cell into the subject.
38-41. (canceled)
Dependencies are inferred from the claim text.