Capillary electrophoresis fluorescent detection system
AssigneeAdvanced Analytical Technologies, Inc.
InventorsHo-ming Pang, Wei Wei
Life of this patent
Estimating the term…
Abstract
The invention includes a high sensitivity and high throughput capillary electrophoresis multiwavelength florescence detection system. The fluorescent detection system is configured to illuminate a relatively large volume of a single capillary or a plurality of capillaries, with a pixelated detection system capable of imaging an area of each capillary that differentiates the capillary walls, the space between the capillaries, and the internal liquid volume within the capillary. Only the desired pixels or image area are used for processing and generating an output signal.
Claims20
A fluorescence detection system, comprising:
- a sample vessel;
- a light source to emit light to excite a fluorescently labeled sample, the system configured to direct light on the sample vessel to illuminate a volume of more than about 100 micrometers×πr2, where r is one-half of the inner diameter of the sample vessel;
- and
- a fluorescence detector positioned to detect the fluorescent emissions of the sample.
A method of detecting a fluorescence emission in a sample the method comprising:
- introducing a fluorescently labeled sample into a sample vessel;
- directing a light from a light source to excite the fluorescently labeled sample, the light directed on the sample vessel to illuminate a volume of more than about 100 micrometers×πr2, where r is one-half of the inner diameter of the sample vessel;
- and
- detecting a fluorescent emission from the fluorescently labeled sample with a fluorescence detector.
A fluorescence detection system, comprising:
- a sample vessel;
- a light source to emit light and to excite a fluorescently labeled sample, the system configured to direct light on the sample vessel to illuminate a volume of more than about 50 micrometers×πr2, where r is one-half of the inner diameter of the sample vessel;
- a fluorescence detector positioned to detect the fluorescent emissions image of the sample, whereby the fluorescence detector has the resolution to detect three distinct regions of the image, including a wall of each capillary, an internal volume of each capillary, and a space between the capillaries, each region being defined by at least one pixel;
- and
- a processor capable of selecting which pixels to process for an integration of light intensity, whereby pixels from the walls of the capillary and pixels from the space between the capillaries are specifically excluded from the integration of the signal.
Dependencies are inferred from the claim text.