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US 2010/0140467 A1Published application
Analysis of amino acids in body fluid by liquid chromatography-mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorScott Goldman
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Abstract
This disclosure provides methods for quantifying individual amino acids in various bodily fluids obtained from a human patient. Also provided are reference ranges for normal amino acid levels in the various bodily fluids (e.g., blood plasma, urine, cerebrospinal fluid, and saliva) and for various age groups (e.g., neonates, infants, children, and adults).
Claims22
A method for identifying the presence of one or more amino acids in a body fluid of an individual, comprising;
- (a) derivatizing one or more amino acids in the body fluid;
- (b) separating the one or more derivatized amino acids by liquid chromatography (LC);
- and
- (c) subjecting the one or more derivatized amino acids from step (b) to mass spectrometry (MS) analysis and quantifying the one or more derivatized amino acid by comparison to structurally similar amino acids from a set of amino acids standards,
- wherein the MS is other than tandem MS.
2 more elements
A method for determining whether a body fluid of an individual contains an abnormal level of one or more amino acids comprising:
- (a) derivatizing amino acids in the body fluid;
- (b) separating the derivatized amino acids by liquid chromatography (LC);
- and
- (c) identifying the derivatized amino acids from step (b) using mass spectrometry (MS) analysis and quantifying the derivatized amino acid by comparison to structurally similar amino acids from a set of amino acids standards, and wherein the MS is other than tandem MS.
1 more element
Dependencies are inferred from the claim text.