Nanoparticle Marker, Diagnostic Methods Using the Same and Diagnostic Kit and Apparatus Using the Same

A method for preparing nanoparticles for labeling a biomaterial, the method comprising the steps of:

• allowing hexadecanol, potassium hydroxide and carbon disulfide to react with each other to prepare a hexadecyl xanthate (HDX) potassium salt;
• allowing the obtained HDX potassium salt to react with one or more nanoparticles selected from a metal group consisting of zinc, cadmium, lead, copper, gallium, arsenic, thallium, nickel, manganese and bismuth so as to prepare HDX metal sulfide nanoparticles;
• and
• allowing the HDX metal sulfide nanoparticles to react with an alkyl amine dopant to prepare metal sulfide nanoparticles.

A diagnostic kit for detecting a specific biomaterial using a nanoparticle label, the diagnostic kit comprising:

• a nanoparticle-biomaterial complex comprising one or more nanoparticles selected from a metal group consisting of zinc, cadmium, lead, copper, gallium, arsenic, thallium, nickel, manganese and bismuth, one or more bio-binding agents binding to the nanoparticles through a binding-stabilizing agent and binding specifically to a biomaterial to be detected, and a binding-stabilizing agent forming bonds between the nanoparticles and the bio-binding agents;
• an extraction solution for isolating and extracting the nanoparticles from the nanoparticle-biomaterial complex;
• a collection electrode for collecting the nanoparticles from the extraction solution;
• and
• a current peak measurement unit for measuring current peaks corresponding to the nanoparticles collected from the collection electrode.

A diagnostic method that uses a nanoparticle label, the method comprising the steps of:

• determining one or more biomaterial-binding materials capable of binding specifically to one or more biomaterials to be detected;
• selecting one or more nanoparticles from the group consisting of zinc, cadmium, lead, copper, gallium, arsenic, thallium, nickel, manganese and bismuth, and binding the selected nanoparticles to the biomaterial-binding materials, respectively, to form one or more nanoparticle-biomaterial complexes;
• mixing the nanoparticle-biomaterial complexes with a sample to be diagnosed, so as to induce the binding between the biomaterials to be detected and the nanoparticle-biomaterial complexes;
• separating the nanoparticle-biomaterial complexes bound to the biomaterials;
• separating the nanoparticles from the separated nanoparticle-biomaterial complexes and collecting the separated nanoparticles;
• and
• measuring characteristic current peaks corresponding to the collected nanoparticles.