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US 2010/0140110 A1Published application
Microfluidic-based lab-on-a-test card for a point-of-care analyzer
AssigneeNanoivd, Inc.
InventorsSunnie Park KIM, Young Shik SHIN, Changgeng LIU, Rory KELLY, Becky CHAN
Life of this patent
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Abstract
A microfluidic-based lab-on-a-test card is described. The test card is used with a point-of-care (POC) analyzer. The test card is designed to receive a sample and then, with the use of the POC analyzer, quantify or count a particular substance in the sample. The test card may be comprised of multiple layers. In one embodiment, the test card includes a primary separation chamber with a filtration surface, a trapping channel, and a particle detector. The test card may also include a nanowire sensor.
Claims48
A device for separating components in a sample, the device comprising:
- a primary separation chamber having a filtration surface defining a plurality of pores;
- a sample inlet in fluid communication with the primary separation chamber, the sample inlet configured to introduce the sample into the primary separation chamber in a direction substantially perpendicular to the filtration surface;
- wherein the filtration surface allows the components smaller than the pores to pass through, and wherein the filtration surface retains components larger than the pores in the primary separation chamber;
- a trapping channel in fluid communication with the primary separation chamber, the trapping channel having walls treated with a capture agent for selectively binding at least one of the larger components that is specifically targeted;
- and
- a buffer inlet in fluid communication with the primary separation chamber, the buffer inlet configured to introduce a buffer into the primary separation chamber in a direction substantially parallel to the filtration surface to sweep the larger components into the trapping channel;
- wherein the walls of the trapping channel selectively bind at least one of the larger components as the larger components pass through the trapping channel.
4 more elements
A method for separating components in a sample, the method comprising:
- providing a primary separation chamber having a filtration surface defining a plurality of pores;
- introducing a sample into the primary separation chamber in a direction substantially perpendicular to the filtration surface, the filtration surface allowing the components smaller than the pores to pass through, the filtration surface retaining components larger than the pores in the primary separation chamber;
- providing a trapping channel in fluid communication with the primary separation chamber, the trapping channel having walls treated with a capture agent for selectively binding at least one of the larger components;
- and
- introducing a buffer in a direction substantially parallel to the filtration surface to sweep the larger components into the trapping channel, the walls of the trapping channel selectively binding at least one of the larger components as the larger components pass through the trapping channel.
2 more elements
A device for separating components in a sample, the device comprising:
- a primary separation chamber having a filtration surface defining a plurality of pores;
- a sample inlet in fluid communication with the primary separation chamber, the sample inlet configured introduce the sample into the primary separation chamber in a direction substantially perpendicular to the filtration surface;
- wherein the filtration surface allows the components smaller than the pores to pass through, and wherein the filtration surface retains components larger than the pores in the primary separation chamber;
- and
- a buffer inlet in fluid communication with the primary separation chamber, the buffer inlet configured to introduce a buffer into the primary separation chamber in a direction substantially parallel to the filtration surface to sweep the larger components off of the filtration surface.
2 more elements
A device for quantifying an amount of a particular substance in a sample, the device comprising:
- a base;
- a source electrode coupled to the base;
- a drain electrode coupled to the base, the drain electrode having a lower potential than the source electrode;
- a semiconducting nanowire coupled to the source electrode and the drain electrode;
- and
- a control electrode coupled to the base, wherein at least a portion of a surface of the control electrode is treated with a capture agent which selectively binds to the substance;
- wherein an amount of current flowing through the nanowire corresponds to a voltage applied to the control electrode, and is affected by an amount of the substance which has bound to the control electrode.
4 more elements
A device for quantifying an amount of a particular substance in a sample, the device comprising:
- a test chamber configured to contain the sample;
- a source electrode;
- a drain electrode, the drain electrode having a lower potential than the source electrode;
- a semiconducting nanowire at least partially positioned within the test chamber, the semiconducting nanowire being coupled to the source electrode and the drain electrode;
- a detector for measuring an amount of current flowing through the nanowire;
- and
- a control electrode at least partially positioned within the test chamber, wherein at least a portion of a surface of the control electrode is treated with a capture agent which selectively binds to the substance;
- wherein an amount of current flowing through the nanowire corresponds to a voltage applied to the control electrode, and is affected by an amount of the substance which has bound to the control electrode.
5 more elements
A device for detecting particles in a sample, the device comprising:
- a main flow channel;
- a buffer introduction channel in fluid communication with the main flow channel, the buffer introduction channel capable of introducing a buffer into the main flow channel to hydrodynamically focus the particles in the sample within the main flow channel;
- a first and a second salt bridge chambers in fluid communication with a first and a second opposing sides, respectively, of the main flow channel, the first and second salt bridge chambers being at least partially filled with agar;
- a first and a second electrolyte chambers in fluid communication with the first and the second salt bridge chambers, respectively, the first and the second electrolyte chambers being at least partially filled with an electrolyte;
- a first and a second electrodes disposed within the first and the second electrolyte chambers, respectively, the first and the second electrodes capable of applying an excitation signal to the first and the second electrolyte chambers and the first and the second salt bridge chambers, respectively;
- an impedance analyzer coupled to the first and second electrodes for measuring an impedance between the first and the second salt bridge chambers;
- and
- wherein particles passing through the main flow channel and between the first and the second salt bridge chambers will cause a detectable change in the impedance between the first and the second salt bridge chambers while the excitation signal is applied.
5 more elements
A method for counting a number of particles in a sample, the method comprising:
- hydrodynamically focusing the particles in a main flow channel;
- applying an excitation signal to a first and a second salt bridge chambers in fluid communication with a first and a second opposing sides, respectively, of the main flow channel, the first and the second salt bridge chambers being filled with agar;
- passing the particles between the first and the second salt bridge chambers;
- and
- counting the number of particles by detecting changes in impedance between the first and the second salt bridge chambers when the particles pass between the first and the second salt bridge chambers.
2 more elements
Dependencies are inferred from the claim text.