Use of plant chromatin remodeling genes for modulating plant architecture and growth
AssigneeWageningen Universiteit
InventorsLudmila Mlynárová, Ton Bisseling
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Abstract
The present invention relates to the field of transgenic plants having modified growth and to the use of chromatin remodeling genes, in particular Arabidopsis gene (AtCHR12) and homologs and orthologs thereof, for making such plants. AtCHR12 is involved in the flexible modulation of growth characteristics in development, notably after the perception of environmental stress. It demonstrates the intimate relationships between environment, chromatin and growth in plants.
Claims13
A transgenic plant or plant part comprising a chimeric gene integrated in its genome, characterized in that said chimeric gene comprises a transcription regulatory sequence active in plant cells operably linked to a nucleic acid sequence selected from the group consisting of:
- (a) a nucleic acid sequence encoding a protein of SEQ ID NO:
- 1;
- (b) a nucleic acid sequence encoding a protein having at least 70% amino acid identity to SEQ ID NO:
- 1 over the entire length;
- (c) a sense and/or antisense fragment of the sequence of (a) or (b),
- wherein said plant or plant part is modified in its growth during exposure to one or more biotic and/or abiotic stresses compared to a non-transgenic plant or plant part.
The plant according to any one of the preceding claims, wherein said transcription regulatory sequence is selected from the group consisting of:
- a constitutive promoter, a inducible promoter, a tissue-specific promoter and a developmentally regulated promoter.
The plant according to any one of the preceding claims, wherein the plant is selected from a genus of the group consisting of:
- Zea, Oryza, Triticum, Lycopersicon, Solanum, Hordeum, Brassica, Glycine, Phaseolus, Avena, Sorghum, Gossypium, Beta, Lactuca, Daucus, Apium, Ipomoea Manihot, Colocasia, Raphanus, Dioscorea, Helianthus and Stachys.
A seed, underground storage organ, fruit, leaf or flower of a plant according to any one of the preceding claims and comprising the chimeric gene.
A chimeric gene comprising a promoter active in plant cells, operably linked to a nucleic acid sequence a nucleic acid sequence selected from the group consisting of:
- (a) a nucleic acid sequence encoding a protein of SEQ ID NO:
- 1;
- (b) a nucleic acid sequence encoding a protein having at least 98% amino acid identity to SEQ ID NO:
- 1 over the entire length;
- (c) a sense and/or antisense fragment of the sequence of (a) or (b).
Use of a nucleic acid sequence encoding a chromatin remodeling protein for the generation of transgenic plants or plant parts having modified growth characteristics, characterized in that the nucleic acid sequence is selected from the group consisting of:
- (a) a nucleic acid sequence encoding a protein of SEQ ID NO:
- 1;
- (b) a nucleic acid sequence encoding a protein having at least 70% amino acid identity to SEQ ID NO:
- 1 over the entire length;
- (c) a fragment of at least 15 consecutive nucleotides of the sequence of (a) or (b).
Use according to claim 13, wherein the modified growth characteristics are one or more of the group consisting of:
- (a) biotic and/or abiotic stress-dependent growth arrest or retardation;
- (b) dormancy-like growth arrest of underground storage organs;
- (c) delayed or suppressed bolting of leaf vegetables;
- and
- (d) embryo growth arrest during seed maturation or seed dormancy.
A method for identifying genes involved in plant growth retardation, growth arrest or dormancy comprising the steps of:
- (a) generating a transgenic plant or plant part which express the protein of SEQ ID NO:
- 1 or a protein comprising at least 70% amino acid identity to SEQ ID NO:
- 1 over the entire length and which is modified in its growth during exposure to one or more biotic and/or abiotic stresses compared to a non-transgenic plant or plant part;
- and
- (b) identifying genes or gene transcripts which are differentially expressed in one or more tissues of the transgenic plants of (a) compared to non-transgenic controls.
Dependencies are inferred from the claim text.