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US 2010/0138957 A1Published application
Hybrid peptides having antimicrobial activity and methods of making and using hybrid peptides
OwnerZhijian T. Li, Dennis J Grayinventors
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OwnerZhijian T. Li, Dennis J Grayinventors
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The present invention concerns the development and utilization of hybrid lytic peptides derived from non-venomous molecular sources to confer a high level of sustainable resistance to phytopathogens in transgenic plants. In an exemplified embodiment, a composition of the invention comprises a cecropin-pleurocidin hybrid peptide of 27 amino acids. The peptide was designed based on optimization of critical molecular and physiochemical parameters. Peptides of the invention offer significantly enhanced antimicrobial activity and molecular properties associated with low cytotoxicity. Transgenic plants of grapevine (Vitis vinifera) that express a peptide of the invention show antimicrobial activity against xylem-limited phytopathogenic bacterium Xylella fastidiasa at a level significantly higher than that from other existing lytic peptides. Thus, the hybrid peptides of the invention can be utilized as an antimicrobial agent for agricultural use.
A peptide comprising an amino acid sequence of a cecropin A peptide and a modified amino acid sequence of a pleurocidin peptide.
A polynucleotide comprising a nucleotide sequence encoding a peptide comprising an amino acid sequence of a cecropin A peptide and a modified amino acid sequence of a pleurocidin peptide.
A method for providing a plant with resistance to a plant pathogen, said method comprising incorporating a polynucleotide into said plant, wherein said polynucleotide comprises a nucleotide sequence encoding a peptide comprising an amino acid sequence of a cecropin A peptide and a modified amino acid sequence of a pleurocidin peptide.
A cell comprising a polynucleotide comprising a nucleotide sequence encoding a peptide comprising an amino acid sequence of a cecropin A peptide and a modified amino acid sequence of a pleurocidin peptide, or comprising a peptide comprising an amino acid sequence of a cecropin A peptide and a modified amino acid sequence of a pleurocidin peptide.
A plant or plant tissue comprising a cell, wherein said cell comprises a polynucleotide comprising a nucleotide sequence encoding a peptide comprising an amino acid sequence of a cecropin A peptide and a modified amino acid sequence of a pleurocidin peptide, or said cell comprises a peptide comprising an amino acid sequence of a cecropin A peptide and a modified amino acid sequence of a pleurocidin peptide.
A method for treating or preventing infection, or providing resistance to a pathogen, in a person or animal, said method comprising administering an effective amount of a peptide comprising an amino acid sequence of a cecropin A peptide and a modified amino acid sequence of a pleurocidin peptide, or a polynucleotide comprising a nucleotide sequence encoding a peptide comprising an amino acid sequence of a cecropin A peptide and a modified amino acid sequence of a pleurocidin peptide to said person or animal.
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Dependencies are inferred from the claim text.