Novel maize plant
AssigneeSyngenta Participations AG
InventorMichel RAGOT
Life of this patent
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Abstract
The present invention relates to maize plants with a genome comprising a unique allele profile associated with the corresponding QTLs contributing to the expression of a variety of phenotypic traits of economic interest selected from the group of grain yield, grain moisture at harvest, early and late root lodging, stalk lodging, common smut incidence, fusarium ear rot incidence, sulcotrione resistance, and tassel architecture. The invention further relates to method for obtaining such a plant as well as assays and screening methods for identifying plants with the desired profile.
Claims35
1-42. (canceled)
A maize plant containing a nuclear genome comprising a set of favourable alleles at a corresponding set of at least 4 QTLs each of which contribute to the phenotypic trait of grain yield, wherein
- a) each QTL is genetically linked to at least one marker locus selected from the group of loci characterized by at least one pair of linked markers each of which can be identified by a pair of PCR oligonucleotide primers consisting of a forward primer and a reverse primer exhibiting a nucleotide sequence as given in
- SEQ ID NO:
- 59/60 and 77/78, respectively, identifying a marker pair linked to QTL1;
- SEQ ID NO:
- 77/78 and 27/28, respectively, identifying a marker pair linked to QTL2
- SEQ ID NO:
- 47/48 and 75/76, respectively, identifying a marker pair linked to QTL3;
- SEQ ID NO:
- 65/66 and 9/10, respectively, identifying a marker pair linked to QTL4;
- SEQ ID NO:
- 69/70 and 13/14, respectively, identifying a marker pair linked to QTL5;
- SEQ ID NO:
- 73/74 and 25/26, respectively, identifying a marker pair linked to QTL6;
- SEQ ID NO:
- 35/36 and 63/64, respectively, identifying a marker pair linked to QTL7;
- SEQ ID NO:
- 35/36 and 63/64, respectively, identifying a marker pair linked to QTL8;
- SEQ ID NO:
- 35/36 and 63/64, respectively, identifying a marker pair linked to QTL9;
- SEQ ID NO:
- 41/42 and 49/50, respectively, identifying a marker pair linked to QTL10;
- SEQ ID NO:
- 49/50 and 61/62, respectively, identifying a marker pair linked to QTL11;
- SEQ ID NO:
- 17/18 and 51/52, respectively, identifying a marker pair linked to QTL12;
- SEQ ID NO:
- 51/52 and 19/20, respectively, identifying a marker pair linked to QTL13;
- and
- SEQ ID NO:
- 29 and 30 identifying a marker linked to QTL14;
- and
- b) each allele at the corresponding QTL is defined by a PCR amplification product, which is essentially identical to the corresponding amplification product of the favourable allele as indicated in Table A obtainable from inbred lines M3047/1 (NCIMB 41459) and M3047/2 (NCIMB 41460) in a PCR reaction using the primer pairs as identified in a).
A maize plant according to any of the preceding claims, wherein said maize plant further comprises 11 QTLs which are contributing to grain moisture mapping to loci on chromosome 1, 2, 3, 4, 5, 7 and 8, wherein each allele at the corresponding QTL is defined by at least one marker allele at said at least one marker locus linked to the QTL, which marker allele is characterized by the PCR amplification product of the respective oligonucleotide primer pair given in Table B, which amplification product is essentially identical to the corresponding amplification product of the favorable allele as indicated in Tables A-G obtainable from inbred lines M3047/1 (NCIMB 41459) and M3047/2 (NCIMB 41460) in a PCR reaction with the identical primer pair.
A set of markers comprising a pair of PCR oligonucleotide primers consisting of a forward primer and a reverse primer capable of identifying a marker linked to a QTL contributing to grain yield, which primers exhibit a nucleotide sequence as given in:
- SEQ ID NO:
- 59/60 and 77/78, respectively, identifying a marker pair linked to QTL1;
- SEQ ID NO:
- 77/78 and 27/28, respectively, identifying a marker pair linked to QTL2
- SEQ ID NO:
- 47/48 and 75/76, respectively, identifying a marker pair linked to QTL3;
- SEQ ID NO:
- 65/66 and 9/10, respectively, identifying a marker pair linked to QTL4;
- SEQ ID NO:
- 69/70 and 13/14, respectively, identifying a marker pair linked to QTL5;
- SEQ ID NO:
- 73/74 and 25/26, respectively, identifying a marker pair linked to QTL6;
- SEQ ID NO:
- 35/36 and 63/64, respectively, identifying a marker pair linked to QTL7;
- SEQ ID NO:
- 35/36 and 63/64, respectively, identifying a marker pair linked to QTL8;
- SEQ ID NO:
- 35/36 and 63/64, respectively, identifying a marker pair linked to QTL9;
- SEQ ID NO:
- 41/42 and 49/50, respectively, identifying a marker pair linked to QTL10;
- SEQ ID NO:
- 49/50 and 61/62, respectively, identifying a marker pair linked to QTL11;
- SEQ ID NO:
- 17/18 and 51/52, respectively, identifying a marker pair linked to QTL12;
- SEQ ID NO:
- 51/52 and 19/20, respectively, identifying a marker pair linked to QTL13;
- SEQ ID NO:
- 29 and 30 identifying a marker linked to QTL14
- which primers lead to an amplification product in a PCR reaction exhibiting a molecular weight or a nucleotide sequence, which is essentially identical to that of a corresponding PCR amplification product obtainable from inbred lines M3047/1 (NCIMB 41459) and M3047/2 (NCIMB 41460) in a PCR reaction with the identical primer pair.
Dependencies are inferred from the claim text.