Method for Producing Stem Cells or Stel Cell-Like Cells from Mammalian Embryos
OwnerIvan Vassiliev, Paul John Vermainventors
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods and compositions for the production and derivation of pluripotent stem cells from embryos or embryo-derived cells and therapeutic uses therefor. In particular, the present invention relates to a method for producing functional stem cells or stem cell-like cells comprising the steps of culturing an embryo or embryo-derived cells in the presence of a demethylation agent and isolating functional pluripotent cells.
Claims23
A method for producing stem cells or stem cell-like cells comprising:
- (i) providing a culture comprising a mammalian embryo or embryo-derived mammalian cells on a feeder layer of cells;
- (ii) introducing to said culture at least one demethylation agent;
- and
- (iii) isolating pluripotent cells.
A method for creating a normal non-human animal comprising the steps of:
- (a) culturing a mammalian embryo or embryo-derived cells in the presence of a demethylation agent;
- (b) isolating pluripotent cells;
- (c) introducing said pluripotent cells into a blastocyst;
- (d) implanting the blastocyst of (c) into a surrogate mother;
- and
- (e) allowing the offspring to develop and be born.
A method for isolating and propagating pluripotent cells comprising the steps of:
- (a) obtaining an embryo or embryo-like cells from a mammal;
- (b) culturing said embryo or embryo-like cells in the presence of at least one demethylation agent;
- (c) recovering said pluripotent cells;
- and
- (d) culturing said pluripotent cells under expansion conditions to produce an expanded cell population.
A method for differentiating pluripotent cells ex vivo comprising the steps of:
- (a) obtaining an embryo or embryo-like cells from a mammal;
- (b) culturing said embryo or embryo-like cells in the presence of at least one demethylation agent;
- (c) recovering said pluripotent cells;
- (d) culturing said pluripotent cells under expansion conditions to produce an expanded cell population;
- and
- (e) culturing the expanded cell population in the presence of desired differentiation factors.
A method for differentiating pluripotent cells in vivo comprising the steps of:
- (a) obtaining an embryo or embryo-like cells from a mammal;
- (b) culturing said embryo or embryo-like cells in the presence of at least one demethylation agent;
- (c) recovering said pluripotent cells;
- (d) culturing said pluripotent cells to produce an expanded cell population;
- and
- (e) administering the expanded cell population to a mammalian host, wherein said cell population is engrafted and differentiated in vivo in tissue specific cells, such that the function of a cell or organ, defective due to injury, genetic disease, acquired disease or iatrogenic treatments, is augmented, reconstituted or provided for the first time.
A therapeutic composition comprising pluripotent cells and a pharmaceutically acceptable carrier, wherein the pluripotent cells are present in an amount effective to produce tissue selected from the group consisting of bone marrow, blood, spleen, liver, lung, intestinal tract, eye, brain, immune system, bone, connective tissue, muscle, heart, blood vessels, pancreas, central nervous system, kidney, bladder, skin, epithelial appendages, breast-mammary glands, fat tissue, and mucosal surfaces including oral esophageal, vaginal and anal and wherein said pluripotent cells are produced by culturing an embryo or embryo-derived cells in the presence of at least one demethylation agent.
A therapeutic method for restoring organ, tissue or cellular function to a mammalian animal in need thereof comprising the steps of:
- (a) obtaining an embryo or embryo-like cells from a mammal;
- (b) culturing said embryo or embryo-like cells in the presence of at least one demethylation agent;
- (c) recovering said pluripotent cells;
- and
- (d) administering the pluripotent cells to the mammalian animal, wherein organ, tissue or cellular function is restored.
Dependencies are inferred from the claim text.