Method of producing a multichimeric mouse and applications to study the immunopathogenesis of human tissue-specific pathologies
AssigneesInstitut Pasteur, Centre National De La Recherche Scientifique, Universite Paris Descartes
InventorsSylvie GARCIA, Eric TARTOUR
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Abstract
The invention relates to a method of producing a multichimeric mouse comprising a functional xenogenic (human) immune system restricted to the MHC class I and/or class II molecules (HLA molecules) of the xenogenic species solely, and a functional tissue. The invention relates also th the use of the multichimeric mouse obtainable by said method, to study the immunopathogenesis of tissue-specific diseases (infectious, tumoral or auto-immune pathologies) and to their applications to design and test vaccines or immunotherapeutic agents against these pathologies.
Claims32
1) A method of producing a multichimeric mouse, characterized in that it comprises the step of:
- transplanting precursor cells of a xenogenic species into a transgenic mouse, by any appropriate means, wherein:
- a) the precursor cells comprise hematopoietic and non-hematopoietic precursors, and
- b) the transgenic mouse has a phenotype comprising :
- b1) a deficiency for murine T lymphocytes, B lymphocytes and NK cells,
- b2) a deficiency for murine MHC class I and MHC class II molecules, and
- b3) a functional MHC class I transgene and/or a functional MHC class II transgene from the same species as the precursor cells.)
Dependencies are inferred from the claim text.