US 2010/0138935 A1 · Detection of cellular stress

Abstract

We provide reporter gene constructs incorporating nucleic acid sequences from a promoter region of a gene or set of genes whose expression is modified in response to external or internal changes in the cellular environment such as biochemical stress or toxic conditions. The promoters are operatively linked to nucleic acid sequences chosen on the basis of the ease with which their transcription and/or translation products may be assayed so that the reporter gene constructs can provide a system capable of detecting intracellular conditions. Also described are transfected cell lines including the constructs and transgenic non-human animal models including the constructs.

Claims33

Dependent claims

A nucleic acid construct comprising:

(i) a nucleic acid sequence of a promoter region of a gene or set of genes whose expression is modified in response to an adverse extracellular or intracellular change in the cellular environment;
(ii) a nucleic acid sequence comprising a transcriptional start site;
and
(iii) at least one or more nucleic acid sequences capable of acting as a template for a defined RNA transcript and providing a read-out in the fog in of an excretable protein and/or an agent capable of being detected histologically.

1 more element

Dependencies are inferred from the claim text.

Detection of cellular stress

Life of this patent

Detection of cellular stress

Life of this patent

Abstract

Claims33