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US 2010/0137579 A1Published application
Process for purifying medical grade hyaluronic acid
AssigneeSustineo Biotechnology Co., Ltd
InventorsWei-Chih Huang, Teh-Liang Chen, Wen-Teng Wu
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Abstract
A process for purifying medical grade hyaluronic acid from a biological source under continual changed pH value is disclosed.
Claims12
A process for purifying medical grade hyaluronic acid from a biological source comprises:
- a) centrifugating a fermentation broth and retaining a supernatant;
- b) adjusting pH value of the supernatant liquid to acidity and then microfiltrating the liquid;
- c) adjusting pH value of a filtrate of step c) to neutral on a reservoir;
- d) diafiltrating the filtrate by acidic solution and ultrafiltrating the filtrate on the reservoir under a through membrane pressure until the filtrate becoming acidity;
- e) adjusting diafiltrate to basicity;
- f) diafiltrating and ultrafiltrating under the through membrane pressure the filtrate on the reservoir until the filtrate becoming neutral;
- and
- g) diafiltrating the HA solution with water.
5 more elements
A process for purifying medical grade hyaluronic acid from a biological source comprises:
- a) centrifugating a fermentation broth and retaining a supernatant;
- b) adjusting pH value of the supernatant liquid to acidity and then microfiltrating the liquid;
- c) adjusting pH value of a filtrate of step c) to 7.0 on a reservoir;
- d) diafiltrating the filtrate by pH 3.5, 1.5 M NaCl and ultrafiltrating the filtrate on the reservoir under a through membrane pressure until the pH value of filtrate reaching 4.0;
- e) adjusting pH value of diafiltrate to 8.0;
- f) diafiltrating and ultrafiltrating the filtrate under the through membrane pressure on the reservoir until the pH value of filtrate reaching 7.0;
- and
- g) diafiltrating the HA solution with water.
5 more elements
Dependencies are inferred from the claim text.