Use of one or more metal carriers to selectively kill mammalian cells
AssigneeCanCure Laboratories, LLC
InventorZoltan KISS
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Abstract
Compositions and methods for decreasing the viability of cells, particularly aberrant non-healthy cells, and most particularly cancer cells, are disclosed. The primary agent that causes cell death is a toxic metal atom or ion. Embodiments of the invention provide compositions and methods to ensure that the toxic metal is directed to the desired cell or tissue. In one embodiment, the metal is bound to a sulfur-rich peptide or protein carrier containing a targeting moiety. Such metal-protein complex is targeted to the selected cells or tissues to enrich the cell or tissue site with the metal-containing peptide or protein molecules followed by administering a dithiocarbonyl which carries the metal from the protein inside the cells to induce cell death.
Claims20
A pharmaceutical composition to induce cancer cell death in tumors, the pharmaceutical composition comprising:
- a metal ion bound to a dithiocarbonyl compound conjugated with one or more targeting molecules, wherein the dithiocarbonyl compound is represented by the formula:
- (R1)m(R2)Z—C—(S)—S—Y
- wherein:
- m is 0 or 1;
- R1 and R2 may be independently hydrogen or C1-C24 straight, branched, or cyclic alkyl, alkenyl, aryl, alkaryl, aralkyl, or alkoxy fragments, said fragments optionally substituted with ester, ether, halogen, sulfate, hydroxyl, or phosphate groups or wherein R1 or R2 may be optionally connected via a bridge comprising —(CH2)n—, wherein n is 3-8, and wherein said bridge may be optionally substituted with C1-C10 straight, branched, or cyclic alkyl, aryl, aryalkyl, or alkaryl groups, each of said groups optionally substituted with hydroxyl, halo, phosphate, sulfate, or sulfonate groups;
- Z is oxygen or nitrogen with the proviso that if Z is oxygen then m is 0;
- and
- Y is C1-C12 amine or C1-C12 carboxyl conjugated with a targeting moiety;
- wherein the targeted dithiocarbonyl compound releases the metal ion into the intracellular space to affect cell death.
A method of synthesizing 3-aminopropylpiperidine-1-carbodithioate.HCl salt (CCK3) comprising the steps of:
- a) dissolving piperidine in a mixture comprising sodium hydroxide and water to form a piperidine mixture;
- b) cooling the piperidine mixture;
- c) adding CS2 in a drop-wise manner to the cooled piperidine mixture while simultaneously stirring the mixture;
- d) stirring the piperidine mixture after the addition of CS2 is complete to precipitate N-piperidinedithiocarbamic acid from the mixture;
- e) removing impurities from the precipitated N-piperidinedithiocarbamic acid;
- and
- f) mixing a solution comprising the N-piperidinedithiocarbamic acid with 3-chloropropylamine (HCL salt) to form and then precipitate 3-aminopropylpiperidine-1-carbodithioate.HCl salt (CCK3).
Dependencies are inferred from the claim text.