Compositions and methods for scaffold formation
AssigneeRegentis Boimaterials Ltd.
InventorsDror Seliktar, Maya Gonen-Wadmany
Life of this patent
Estimating the term…
Abstract
The present invention relates to scaffolds composed of a protein backbone cross-linked by a synthetic polymer. Specifically, the present invention provides PEGylated-thiolated collagen scaffolds and PEGylated albumin scaffolds and methods of generating and using same for treating disorders requiring tissue engineering.
Claims33
A composition-of-matter comprising a polymer-protein precursor molecule which comprises a thiolated protein and at least two synthetic polymers covalently attached to thiol groups of said thiolated protein, each of said at least two synthetic polymers having a functional group, said functional group being selected capable of cross-linking with a functional group of at least one other synthetic polymer being covalently attached to at least one other polymer-protein precursor molecule so as to form a scaffold.
A scaffold comprising a plurality of thiolated proteins and a plurality of synthetic polymers covalently attached to thiol groups of said thiolated protein, each of said thiolated proteins being covalently attached to at least two of said synthetic polymers, wherein said synthetic polymers are cross-linked.
A hydrogel comprising a plurality of thiolated proteins and a plurality of synthetic polymers covalently attached to thiol groups of said thiolated protein, each of said thiolated proteins being covalently attached to at least two of said synthetic polymers, wherein said synthetic polymers are cross-linked.
A method of generating a scaffold, the method comprising:
- (a) thiolating a protein with a thiolating reagent, to thereby obtain a thiolated protein;
- (b) covalently attaching a thiolated protein to at least two synthetic polymers through a first functional group of said at least two synthetic polymers, wherein said first functional group attaches to a thiol group of said thiolated protein, each of said at least two synthetic polymers having a second functional group, to thereby obtain a polymer-protein precursor molecule;
- and subsequently
- (c) cross-linking a plurality of said precursor molecules to thereby generate said scaffold.
(canceled)
(canceled)
A composition-of-matter comprising a polymer-protein precursor molecule which comprises albumin and at least two synthetic polymers covalently attached to said albumin, each of said at least two synthetic polymers having a functional group, said functional group being selected capable of cross-linking with a functional group of at least one other synthetic polymer being covalently attached to at least one other polymer-protein precursor molecule so as to form a scaffold.
A scaffold comprising a plurality of albumin molecules and a plurality of synthetic polymers covalently attached to said albumin molecules, each of said albumin molecules being covalently attached to at least two of said synthetic polymers, wherein said synthetic polymers are cross-linked.
(canceled)
A method of generating a scaffold, the method comprising:
- (a) covalently attaching albumin to at least two synthetic polymers through a first functional group of said at least two synthetic polymers, each of said at least two synthetic polymers having a second functional group, to thereby obtain a polymer-protein precursor molecule;
- and subsequently
- (b) cross-linking a plurality of said precursor molecules to thereby generate said scaffold.
22-23. (canceled)
26-28. (canceled)
30-32. (canceled)
35-38. (canceled)
41-43. (canceled)
Dependencies are inferred from the claim text.