Lentivirus pseudotyped with influenza hemagglutinin and methods of use
AssigneeInstitut Pasteur of Shanghai
InventorsPaul ZHOU, Cheguo Tsai, Tetsuya Toyoda, Philippe Buchy
Life of this patent
Estimating the term…
Abstract
Highly effective pseudotyping of lentiviral vector with influenza HA, NA and M2 packaging gene constructs at the proper ratios. Lentivirus vector pseudotyped with influenza HA, especially pseudotyped with H5 and neuraminidase. Methods of inducing immune responses to influenza antigens or for transducing genes into cells to which influenza antigens bind using such lentivirus vectors. Methods for screening drugs which inhibit influenza infection using lentivirus pseudotyped with HA.
Claims30
A lentivirus vector pseudotyped with:
- an influenza HA protein or a protein containing an HA protein fragment comprising an HA epitope or an HA cellular attachment ligand,
- wherein said HA protein is not fowl plague virus H7HA.
A lentivirus vector packaging system comprising:
- at least one packaging vector expressing HA, and
- a transfer vector construct comprising production and packaging sequences, sequences expressing the Gag and Pol lentivirus proteins, and optionally a transgene,
- wherein said HA protein is not H7HA.
A lentivirus vector packaging system comprising:
- at least one packaging vector expressing HA,
- a helper construct expressing the Gag and Pol lentivirus proteins, and
- a transfer vector construct comprising production and packaging sequences and optionally a transgene;
- wherein said HA protein is not H7HA.
A pseudotyped Lentivirus vector based neutralization assay comprising the steps of:
- a) bringing into contact a first population of cells with
- at least one Lentivirus vector comprising a marker and pseudotyped with one or more antigens selected from the group:
- an influenza HA protein or a protein containing an HA protein fragment comprising an HA epitope or an HA cellular attachment ligand and an influenza NA protein, a protein containing an NA protein fragment comprising or an NA epitope and
- a sample of sera;
- b) incubating said first population of cells with said at least one pseudotyped Lentivirus vector and said sera;
- c) determining the presence of said marker in said population of cells.
Dependencies are inferred from the claim text.