US 2010/0137159 A1 · Simple Tests for Rapid Detection of Canine Parvovirus Antigen and Antibodies

Abstract

Slide agglutination tests (SATs) and slide inhibition tests (SITs) provide rapid detection, quantitation and strain identification of red blood cell (RBC) agglutinating viruses such as canine parvovirus (CPV) in biological samples. The tests are rapid, low-cost, and easy to use. These tests do not require any expensive equipment and can thus be used to monitor infections and antibody titers under field conditions. The tests can be modified to detect results using fluorescence (FSAT). FSAT is useful for rapid high-throughput screening (HTS) of libraries of small molecules and/or chemical compounds to identify antiviral compounds useful for the treatment of diseases caused by emerging hemaglutinating viruses that infect animals and humans.

Claims15

Dependent claims

A method for detecting the presence of a red blood cell (RBC) agglutinating virus in a sample, comprising the steps of

mixing, on the surface of a substrate, a microliter quantity of said sample with a microliter quantity of a RBC solution to form a reaction mixture with a final volume of 1000 μl or less;
and
incubating said reaction mixture for one minute or less;
and
detecting in said reaction mixture on said surface of said substrate the presence or absence of RBC agglutination, wherein the presence of RBC agglutination indicates the presence of a RBC agglutinating virus in said sample, and the absence of RBC agglutination indicates the absence of a RBC agglutinating virus in said sample.

2 more elements

Dependencies are inferred from the claim text.

Simple Tests for Rapid Detection of Canine Parvovirus Antigen and Antibodies

Life of this patent

Simple Tests for Rapid Detection of Canine Parvovirus Antigen and Antibodies

Life of this patent

Abstract

Claims15