Mutants of Deoxycytidine Kinase Having Extended Enzymatic Activity
AssigneeInstitut Pasteur and Centre National de la Recherche Scientifique (CNRS)
InventorsPhilippe Marliere, Sylvie Pochet, Madeleine Bouzon
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Abstract
The invention relates to a method for artificial in vivo evolution of proteins, said method making it possible to bring about the evolution of a protein X by complementation of a relative protein Y, X and Y both belonging to the same class of enzyme commission (EC) nomenclature or belonging to related classes. The mutants D133E and R104Q of desoxycytidine kinase (DCK) were obtained; both of said mutations result in acquisition of thymidine kinase activity by DCK.
Claims12
1-12. (canceled)
A kinase, which is a human DCK1 possessing the sequence filed in GENBANK under accession number M60527 comprising at least one mutation selected from the mutations D133E and R104Q.
14-30. (canceled)
Dependencies are inferred from the claim text.