Polyvalent RNA-Nanoparticle Compositions
AssigneeNorthwestern University
InventorsChad A. Mirkin, David A. Giljohann, Dwight Seferos, Andrew E. Prigodich, Pinal C. Patel
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Abstract
The present invention concerns nanoparticles functionalized with duplex RNA for a variety of uses, including but not limited to gene regulation. More specifically, the disclosure provides a new strategy for conjugating RNA to a nanoparticle to achieve increased stability and activity.
Claims38
A nanoparticle composition comprising:
- one or more ribonucleic acid (RNA) polynucleotides in association with a nanoparticle;
- the RNA polynucleotide having a polypeptide interaction site;
- the RNA polynucleotide having a sequence that forms a duplex under conditions appropriate to form the duplex;
- the duplex having at least one domain in a single strand of the duplex sufficiently complementary to a sequence in a target polynucleotide to permit hybridization of the single strand to the target polynucleotide under appropriate conditions, and hybridization of the domain of the duplex to the sequence in the target polynucleotide creates a substrate site recognized by a polypeptide;
- and
- the RNA polynucleotide associated with the nanoparticle in an orientation specific manner with respect to the polypeptide interaction site and the nanoparticle.
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A method of associating a RNA polynucleotide to a nanoparticle comprising the step of aging a mixture of a thiolated RNA polynucleotide duplex with the nanoparticle in a series of solutions to associate the RNA polynucleotide to the nanoparticle, each solution comprising an increasing concentration of sodium chloride (NaCl) relative to a previous solution beginning with a first solution comprising about 0.1 M NaCl.
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Dependencies are inferred from the claim text.