Cell/tissue culturing device, system and method
AssigneeProtalix Ltd.
InventorYoseph SHAALTIEL
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Abstract
A device, system and method for axenically culturing and harvesting cells and/or tissues, including bioreactors and fermentors. The device is preferably disposable but nevertheless may be used continuously for a plurality of consecutive culturing/harvesting cycles prior to disposal of same. This invention also relates to batteries of such devices which may be used for large-scale production of cells and tissues. According to preferred embodiments of the present invention, the present invention is adapted for use with plant cell culture.
Claims21
A system for culturing cells and or tissue, the system comprising at least one disposable device for axenically culturing and harvesting cells and/or tissue in at least one cycle, wherein said device comprises a sterilisable disposable container which comprises a reusable harvester which comprises a flow controller for harvesting of at least a desired portion of culture medium containing cells and/or tissues when desired, and wherein said device can be used continuously for at least one further consecutive culturing/harvesting cycle, wherein a remainder of said medium containing cells and/or tissue, remaining from a previous harvested cycle, may serve as inoculant for a next culture and harvest cycle, wherein said cells and/or tissue are plant cells and/or plant tissue and wherein said device comprising at least two air inlets being positioned at or near the bottom end of said device, wherein said bottom of said device is substantially frustro-conical and wherein said air inlets are designed to produce bubbles comprising a mean diameter of between 1 to 10 mm, and wherein said system further comprises a culture of carrot cells expressing a recombinant human lysosomal glucocerebrosidase.
Dependencies are inferred from the claim text.