Generation of oncolytic adenoviruses and uses thereof

A method for isolating an oncolytic adenovirus with enhanced potency toward tumor cells derived from colon, ovarian, lung, prostate, breast or pancreas, when compared with a reference virus, said method comprising

• (a) pooling of a group of adenoviruses, wherein said adenoviruses are selected from the group consisting of the adenoviral serotypes B, C, D, E and F;
• (b) passaging the pooled adenoviral mixture from step (a) on an actively growing culture of tumor cells;
• (c) harvesting the supernatant from step (b);
• (d) infecting a quiescent culture of tumor cells with the supernatant harvested in step (c);
• (e) harvesting the cell culture supernatant from step (d) prior to any sign of CPE;
• (f) infecting a quiescent culture of tumor cells with the supernatant harvested in step (e);
• (g) harvesting the cell culture supernatant from step (f) prior to any sign of CPE;
• and
• (h) isolating a virus from the supernatant of step (g) by plaque purification, wherein the tumor cells in steps (b), (d) and (f) are all grown on, or in, an extracellular matrix.