Loading patent…
AssigneeByoung-Hyun MIN
InventorsByoung-Hyun Min, So Ra Park, Cheng Zhe Jin, Kwideok Park
Estimating the term…
The present invention relates to a method for fabricating a cell-derived extracellular matrix scaffold, more particularly, to a method for fabricating a cell-derived extracellular matrix scaffold, the method comprising the steps of obtaining a chondrocyte/extracellular matrix (ECM) membrane from chondrocytes derived from animal cartilage, obtaining a pellet-type scaffold-free construct by culturing after centrifuging the obtained chondrocytes/extracellular matrix (ECM) membrane and freeze-drying the obtained pellet-type construct. The cell-derived ECM scaffold according to the invention is a porous scaffold fabricated using cartilage tissue engineered by culturing chondrocytes in an in vitro scaffold-free system, which is not reduced in size during the cultivation and thus useful for cartilage regeneration.
The method of claim 15, wherein the method comprising the steps of:
The method of claim 15, wherein the method comprising the steps of:
The method of claim 15, wherein the animal is a pig.
The method of claim 15 further comprising adding a growth factor additionally in step (a), the culture step.
The method of claim 15, wherein culture broth is treated with ultrasonic waves or physical pressure is applied to the culture broth in the culture step.
A cell-derived porous ECM scaffold fabricated by the method of claim 15, which has pores with a diameter of 10˜1000 μm.
A method for fabricating a cell-derived ECM scaffold comprising:
Dependencies are inferred from the claim text.