System for and method of positioning cells and determining cellular activity thereof

A system for measuring electrical activity of a plurality of cells, the system comprising:

• (a) a non-conductive substrate formed with a plurality of addressable pores and a plurality of channels embedded in said substrate and being in fluid communication with said plurality of addressable pores;
• (b) a plurality of multi-electrode-arrays, each one of said plurality of multi-electrode-arrays includes a plurality of electrode structures formed on a first side of said non-conductive substrate and positioned in one of said pores, such that at least one of said electrode structures emerges from a base of said pore and being flush with a surface of said first side;
• (c) a plurality of conductive elements formed on a second side of said non-conductive substrate, wherein each one of said conductive elements is electrically coupled to one of said electrode structures;
• and
• (d) a fluid source being in fluid communication with said plurality of channels;
• said pores, said channels, said electrode structures and said fluid source are designed and constructed so that said electrode structures sense electrical signals from the plurality of cells while said fluid source continuously exchanges fluids with said channels and pores.