In vitro tumor angiogenesis model
AssigneeBecton, Dickinson and Company
InventorMIN WU
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Abstract
Provided is a method of inducing tubulogenesis in normal endothelial cells comprising co-culturing the normal endothelial cells with tumor cells and forming tubules from the normal endothelial cells.
Claims18
An in vitro method of inducing tubulogenesis in normal endothelial cells comprising co-culturing the normal endothelial cells with tumor cells and forming tubules from the normal endothelial cells, wherein the tumor cells are derived from a solid tumor selected from the group consisting of non-small cell lung cancer, prostate cancer, breast cancer, gastric cancer and tumors of the head and neck.
A method of preparing angiogenic normal endothelial cells comprising:
- a) co-culturing normal endothelial cells with tumor cells;
- b) forming tubules from the normal endothelial cells;
- and
- c) selectively collecting the tubules, wherein the tubules comprise angiogenic normal endothelial cells.
A method of identifying bioactive agents comprising:
- a) co-culturing normal endothelial cells with tumor cells, thereby forming tubules from the normal endothelial cells;
- b) contacting the normal endothelial cells with a prospective bioactive agent;
- and
- c) monitoring for changes in tubule formation resulting from the presence of the bioactive agent.
Dependencies are inferred from the claim text.