High-throughput assay for evaluating dipeptidyl peptidase i activity
AssigneeJohnson & Johnson Pharmaceutical Research & Development, L.L.C.
InventorsMatthew Olson, Matthew Todd
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Abstract
A method of screening a compound which modulates dipeptidyl peptidase I (DPPI) activities comprises the steps of adding a peptide substrate of DPPI to a reaction mixture which comprises DPPI and a compound, wherein the peptide substrate of DPPI has at least 3 amino acids and binds to a binding site of DPPI in addition to the S1-S2 site; and measuring the molecular weight of the substrate, wherein a change in the molecular weight of the substrate is indicative of the presence of DPPI activity.
Claims10
A method of screening a compound which modulates dipeptidyl peptidase I(DPPI) activities, comprising the steps of:
- a. adding a substrate of DPPI to a reaction mixture which comprises DPPI and said compound, wherein said substrate of DPPI comprises at least 3 amino acids and binds to a binding site of DPPI in addition to the S1-S2 site;
- and
- b. measuring the molecular weight and/or the change in mass ratio of said substrate and product,
- wherein a decrease in the molecular weight of said substrate is indicative of the presence of said DPPI activity.
A DPPI substrate selected from the group consisting of SEQ ID NOs:
- 1-7.
The DPPI substrate of SEQ ID NO:
- 1
Dependencies are inferred from the claim text.