Photo-Induced Damage Mitigating Agents and Preparation and Methods of Use Thereof
AssigneePacific Biosciences of California, Inc.
InventorsXiangxu Kong, Gene Shen, Andrei Fedorov, John Lyle, Grace Lee, Lubomir Sebo, Duc Do, Robert Weber, Stephen Dudek
Life of this patent
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Abstract
Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photo-induced damage mitigating agents to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.
Claims44
A composition of the invention comprising:
- a first reactant;
- a second reactant;
- and
- a photo-induced damage mitigating agent admixture;
- wherein interaction of the first reactant with the second reactant under excitation illumination causes photo-induced damage to the first reactant in the absence of the photo-induced damage mitigating agent admixture.
9-10. (canceled)
A method of performing an illuminated reaction, comprising:
- providing a substrate having a reaction mixture disposed thereon, wherein the reaction mixture comprises a first reactant, a second reactant, and a photo-induced damage mitigating agent admixture, wherein the photo-induced damage mitigating agent admixture reduces an amount of photo-induced damage to the first reactant resulting from interaction of the first reactant with the second reactant under excitation illumination to an amount that is less than that which would occur in the absence of the photo-induced damage mitigating agent admixture;
- and
- illuminating the reaction mixture on the substrate with an excitation illumination.
22-23. (canceled)
26-35. (canceled)
A compound having a formula selected from the group consisting of
- wherein each R1 is independently selected from the group consisting of hydrogen, alkyl, —CH3, —(CH2)nR2, —(CH2)nR2R3, —OR2, —COR2, —COOR2, —(CH2)nOR2, —CH(OH)R2, —(CH2)nR5, —CH(OR2)R3, —(CH2)nR2R5, —R5, —OR5, —COR5, —COOR5, —(CH2)nOR5, where n is an integer from 1 to 4, and further wherein the compound is linked to a nucleotide polyphosphate, a polymerase, a surface, or a combination thereof.
(canceled)
44-46. (canceled)
48-57. (canceled)
A device comprising:
- a substrate having an observation region;
- a first reactant immobilized within the observation region;
- a second reactant disposed within the observation region, wherein interaction between the first and second reactants under excitation illumination causes photo-induced damage to the first reactant;
- and
- at least one photo-induced damage mitigating agent admixture comprising a compound having a formula selected from the group consisting of
- disposed within the observation region.
59-62. (canceled)
66-79. (canceled)
A photo-induced damage mitigating agent admixture comprising at least about 10% of a compound of the formula
- at least 10% of a compound of the formula
- and no more than about 20% of a compound of the formula
81-90. (canceled)
Dependencies are inferred from the claim text.