Method of measuring lipid in specific lipoprotein
AssigneeDaiichi Pure Chemicals Co., Ltd.
InventorsShoko YAMAMOTO, Mitsuaki Yamamoto, Kazuo Nakanishi, Kazunori Saito
Life of this patent
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Abstract
A method of measuring a lipid in a specific lipoprotein characterized by using a polycyclic polyoxyalkylene derivative at least in the step of determining the specificity of the measurement of the target lipid.
Claims24
A method for assaying a lipid contained in a specific lipoprotein, comprising:
- a specificity determining step in which a polycyclic polyoxyalkylene derivative is employed to determine the specificity of an assay for a target lipid.
A method for assaying triglyceride contained in a specific lipoprotein, which method comprising:
- pretreating free glycerol such that the glycerol does not participate in reaction, and
- applying lipoprotein lipase to act on a specific lipoprotein,
- wherein the lipoprotein lipase is reacted with triglyceride contained in the specific lipoprotein in the presence of a polycyclic polyoxyalkylene derivative having an HLB of 12 to 18.
A method for assaying triglyceride contained in a specific lipoprotein, which method comprising:
- pretreating free glycerol such that the glycerol does not participate in reaction, and
- applying lipoprotein lipase to act on a specific lipoprotein,
- wherein the free glycerol and triglyceride contained in lipoproteins other than the specific lipoprotein are pretreated in the presence of a polycyclic polyoxyalkylene derivative having an HLB of 12 to 15.
A reagent for assaying a lipid contained in a specific lipoprotein, comprising:
- a polycyclic polyoxyalkylene derivative which acts on a specific lipoprotein, and
- a target lipid assay reagent.
The reagent according to claim, wherein the polycyclic polyoxyalkylene derivative is a nonionic or anionic surfactant having two or more aryl groups.
Dependencies are inferred from the claim text.