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US 2010/0136584 A1Published application
Methods for using antibodies and analogs thereof
AssigneeICB International, Inc.
InventorsRam S. Bhatt, Rishi Bhatt
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Abstract
Camelid and shark single-domain heavy chain antibodies lacking the light chains, and their analogs are disclosed. Methods for using the antibodies and their analogs to detect antigens are disclosed. Methods to derivatize such antibodies and analogs to develop diagnostic assays are described. Also provided are kits, and methods of using such diagnostics assays.
Claims21
A method for detecting the presence or absence of an antigen in a sample comprising:
- a) obtaining a sample suspected of having said antigen,
- b) detecting the presence or absence of said antigen in said sample utilizing a polypeptide, wherein said polypeptide comprises all or a portion of at least one variable antigen-binding (Vab) domain of camelid and/or shark single-domain heavy chain antibodies lacking light-chains, at least ten contiguous amino acids derived from a source other than camelid and/or shark single-domain heavy chain antibodies lacking light-chains, wherein said polypeptide comprises at least one binding site for an antigen, wherein said polypeptide binds specifically to said antigen, wherein said binding is indicative of the presence of said antigen.
A method for diagnosing an individual with a disease, said method comprising:
- a) obtaining a sample from said individual
- b) detecting the presence or absence of one or more biomarkers associated with said disease, wherein said detection comprises utilizing a polypeptide, wherein said polypeptide comprises all or a portion of at least one variable antigen-binding (Vab) domain of camelid and/or shark single-domain heavy chain antibodies lacking light-chains, at least ten contiguous amino acids derived from a source other than camelid and/or shark single-domain heavy chain antibodies lacking light-chains, wherein said polypeptide binds specifically to at least one of said biomarkers;
- and said binding of said polypeptide to one or more of said biomarkers is indicative of the presence of said one or more biomarkers in said sample,
- c) identifying said individual as having said disease when said one or more biomarkers are present in said individual's sample.
1 more element
A method for detecting the presence or absence of circulating cells in a sample comprising
- a) obtaining a sample suspected of having circulating cells,
- b) detecting the level of one or more antigen associated with said circulating cell in said sample utilizing a polypeptide, wherein said polypeptide comprises all or a portion of at least one variable antigen-binding (Vab) domain of camelid and/or shark single-domain heavy chain antibodies lacking light-chains, at least ten contiguous amino acids derived from a source other than camelid and/or shark single-domain heavy chain antibodies lacking light-chains, wherein said polypeptide binds specifically to said one or more antigens, and wherein said binding of said polypeptide to said antigens is indicative of the presence of circulating cells in said sample.
Dependencies are inferred from the claim text.