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US 2010/0136569 A1Published application
Compositions, methods and kits for polynucleotide amplification reactions and microfluidic devices
AssigneeCepheid
InventorsByung Sook Moon, Jose Johnny Valdez
Life of this patent
Estimating the term…
Abstract
Antifoam agents improve detection of polynucleotide amplification reactions and improve manipulation of fluids in microfluidic devices.
Claims20
A reagent formulation, comprising
- at least one reagent for polynucleotide amplification or detection;
- and
- an antifoam agent.
A microfluidic device containing a mixture, wherein the mixture comprises
- at least one reagent for amplifying or detecting a polynucleotide;
- and
- an antifoam agent.
A method of detecting the product of an amplification reaction, the method comprising,
- performing an amplification reaction in a mixture comprising an antifoam agent;
- and
- detecting the product of the amplification reaction.
A method of improving optical detection in a microfluidic device, the method comprising,
- providing in the microfluidic device a mixture comprising an antifoam agent;
- and
- detecting a component of the mixture.
Dependencies are inferred from the claim text.