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US 2010/0136559 A1Published application
Chromatin structure detection
AssigneeBio-Rad Laboratories, Inc.
InventorsSteve Okino, Man Cheng
Life of this patent
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Abstract
The present application provides methods and compositions for determining accessibility of a DNA modifying agent in genomic DNA.
Claims21
A method for analyzing chromosomal DNA, the method comprising,
- a. simultaneously:
- i. permeabilizing or disrupting a cell membrane of a cell;
- and
- ii. contacting the cell with a DNA cleaving or modifying agent under conditions such that the agent cleaves or modifies the genomic DNA in the cell, wherein different regions of the genomic DNA are cleaved or modified to a different extent by the agent, thereby generating cleaved and intact DNA regions, or modified and unmodified DNA regions;
- and
- b. detecting a quantity of at least one intact or unmodified or modified DNA region or cloning, isolating, or nucleotide sequencing at least one intact or unmodified or modified DNA region.
3 more elements
A kit for determining the accessibility of a locus on a chromosome to a DNA modifying agent, the kit comprising,
- a cell membrane permeabilizing or disrupting agent;
- a restriction enzyme or a DNase.
Dependencies are inferred from the claim text.