Reproducible quantification of biomarker expression
AssigneeHistoRx, Inc.
InventorsJason Christiansen, Robert Pinard, Mark Gustavson, Brian Bourke, Gregory R. Tedeschi, Dylan M. Reilly, Maciej P. Zerkowski, Christine (Jones) Williams, Dongxiao Wang
Life of this patent
Estimating the term…
Abstract
A method is described for the reproducible quantification of biomarker expression, including biomarker expression in a tissue sample. Methods and systems are described whereby reproducible scores for biomarker expression are obtained independent of instrument, its location, or operator.
Claims25
A method of reproducibly quantifying biomarker expression in a slide-mounted tissue sample comprising:
- (a) obtaining a slide-mounted tissue sample, which has been stained to permit localization of at least one cellular compartment and at least one biomarker;
- (b) obtaining one or more pixel-comprised images of the stained tissue sample using a standardized optical system that includes a light source;
- (c) automatically analyzing one or more data sets derived from the image pixels to differentiate data signal from noise;
- (d) automatically analyzing one or more data sets derived from the image pixels to differentiate data signal attributable to each of said at least one cellular compartment;
- (e) optionally automatically analyzing one or more data sets derived from the image pixels to differentiate data signal attributable to said at least one biomarker for each of said at least one cellular compartment;
- (f) quantifying the amount of biomarker expressed in each of said at least one cellular compartment,
- whereby the biomarker expression in the slide-mounted tissue sample is quantified reproducibly.
A computer readable medium having computer readable instructions stored thereon for execution by a processor to perform a method of reproducibly quantifying biomarker expression in a slide-mounted tissue sample comprising:
- (a) acquiring one or more pixel-comprised images of a slide-mounted tissue sample, which has been stained to permit localization of at least one cellular compartment and at least one biomarker using a standardized optical system that includes a light source;
- (b) automatically analyzing one or more data sets derived from the image pixels to differentiate data signal from noise;
- (c) automatically analyzing one or more data sets derived from the image pixels to differentiate data signal attributable to each of said at least one cellular compartment;
- and
- (d) quantifying the amount of biomarker expressed in each of said at least one cellular compartment,
- whereby the biomarker expression in the slide-mounted tissue sample is quantified reproducibly.
A system for reproducibly quantifying biomarker expression in a slide-mounted tissue sample comprising:
- (a) one or more lenses configured to magnify at least a portion of a slide-mounted tissue sample, which has been stained to permit localization of at least one cellular compartment and at least one biomarker;
- (b) a standardized optical system, including a light source and an image sensor in optical communication with said one or more lenses, the standardized optical system obtaining one or more pixel-comprised images of the stained tissue sample;
- (c) a processor module in communications with the standardized optical system, the processor module configured to:
- (i) automatically analyze one or more data sets derived from the image pixels to differentiate data signal from noise, (ii) automatically analyze one or more data sets derived from the image pixels to differentiate data signal attributable to each of said at least one cellular compartment, and (iii) quantify the amount of biomarker expressed in each of said at least one cellular compartment, whereby the biomarker expression in the slide-mounted tissue sample is quantified reproducibly.
Dependencies are inferred from the claim text.