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US 2010/0136538 A1Published application
Marking reagents bearing diazo and nitro functions, methods for the synthesis of such reagents and methods for detecting biological molecules
AssigneesBiomerieux, Cnrs, Universite De Strasbourg
InventorsAlain Laurent, Ali Laayoun, Mitsuharu Kotera
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Abstract
The present invention relates to a labeling reagent of formula: in which: R1 represents at least one detectable label, L and A are each a linker arm, n is an integer equal to 1, and u is an integer between 0 and 2. The present invention also describes a method of synthesizing said markers and also applications for the labeling of biological molecules, more particularly nucleic acids, with a labeling reagent bearing diazo and nitro functions. The invention is particularly suitable for use in the diagnostics field.
Claims17
A labeling reagent of formula (A):
- in which:
- R1 represents a detectable label or at least two detectable labels linked to one another by at least one multimeric structure,
- R2 and R3 represent, independently of one another:
- H, NO2, Cl, Br, F, I, R4-(L)n-Y—X—, OR, SR, NR2, R, NHCOR, CONHR or COOR, with R being alkyl or aryl, and R4 represents a detectable label or at least two detectable labels linked to one another by at least one multimeric structure,
- L is a linker arm comprising a linear chain of at least two covalent bonds,
- n is an integer equal to 1,
- A is a linker arm comprising at least one covalent double bond allowing the conjugation of the diazo function with the aromatic ring, and
- u is an integer between 0 and 2.
5 more elements
Dependencies are inferred from the claim text.