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AssigneeNational Taiwan University
InventorsJin-Town Wang, Yi-Jiun Pan, Han-Chi Fang, Hui-Ching Yang, Tzu-Lung Lin, Pei-Fang Hsieh, Feng-Chiao Tsai, Yoav Keynan
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The present invention relates to a method of identifying a serotype of Klebsiella pneumoniae, in particular to a method using specific polymerase chain reaction (PCR) primer sets designed according to a fragment of a capsular polysaccharide synthesis (cps) region to identify a K57 or a NTUH-N1 serotype and its application. NTUH-N1 is a novel serotype which differs from the previously reported 77 serotypes. This PCR-based cps genotyping method not only solves the problems of insufficient specificity and sensitivity caused by conventional immune method, but can be applied in clinical diagnosis with the advantages of rapidity and low cost. In addition, the rate of unidentifiable strains can also be reduced by this method.
A method of identifying a serotype of Klebsiella pneumoniae, the method comprising:
A method of identifying a serotype of Klebsiella pneumoniae, the method comprising:
A primer set of identifying a NTUH-N1 serotype of Klebsiella pneumoniae comprising DNA of a NTUH-N1 serotype of Klebsiella pneumoniae for use as a template to obtain to a fragment of a capsular polysaccharide synthesis (cps) region of the NTUH-N1 serotype of Klebsiella pneumoniae through PCR, wherein the primer set is selected from the group consisting of a third primer set (SEQ ID NO:25 and SEQ ID NO:26), a forth primer set (SEQ ID NO:27 and SEQ ID NO:28), a fifth primer set (SEQ ID NO:29 and SEQ ID NO:30), a sixth primer set (SEQ ID NO:31 and SEQ ID NO:27), a seventh primer set (SEQ ID NO:32 and SEQ ID NO:33), and a eighth primer set (SEQ ID NO:34 and SEQ ID NO:35).
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