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US 2010/0136527 A1Published application
Dominant B Cell Epitopes and Methods of Making and Using Thereof
AssigneesThe Regents of the University of California, National Institutes of Health
InventorGang Zeng
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Abstract
Disclosed are methods for obtaining at least one epitope suitable for detecting the presence of an antibody against a tumor associated antigen of a cancer in a sample. Kits, assays, and substrates employing the epitopes of the present invention are disclosed. Also disclosed are epitopes of NY-ESO-1 and XAGE-1b and methods of using thereof.
Claims17
An isolated polypeptide
- (a) comprising SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, or SEQ ID NO:10;
- (b) consisting essentially SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, or SEQ ID NO:10;
- (c) consisting of SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, or SEQ ID NO:10;
- (d) comprising at least 20 consecutive amino acid residues of SEQ ID NO:15;
- (e) consisting essentially of at least 20 consecutive amino acid residues of SEQ ID NO:15;
- (f) consisting of at least 20 consecutive amino acid residues of SEQ ID NO:15;
- (g) consisting essentially of SEQ ID NO:16 or SEQ ID NO:17;
- or
- (h) consisting of SEQ ID NO:16 or SEQ ID NO:17.
6 more elements
A method for obtaining at least one epitope suitable for detecting the presence of at least one antibody against a tumor associated antigen of a cancer which comprises
- selecting at least one tumor associated antigen known to be expressed by the cancer,
- predicting the hydrophilicity and surface accessibility of a plurality of peptides having about 9 to about 12 consecutive amino acid residues of the tumor associated antigen,
- screening the peptides having a desired hydrophilicity and surface accessibility against antibodies that specifically bind the tumor associated antigen, and
- detecting the peptides which specifically bind the antibodies.
1 more element
Dependencies are inferred from the claim text.