US 2010/0136525 A1Published application

Fluid analysis device and method

AssigneeKoninklijke Philips Electronics N.V.

InventorsJacobus Frederik Molenaar, Adrianus Wilhelmus Dionisius Maria Van Den Bijgaart

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Abstract

The invention provides an analysis device (1) and method, that enables repeatedly pumping a sample fluid (50) with one or more analytes through a substrate (10) with one or more binding materials, even when the wetted substrate has a relatively low bubble pressure. Thereto, the device (1) comprises a first volume (16) and a second volume (18), and a return channel (22) with a valve (24) as well as a pump (20). The pump (20) can establish a pressure difference between the first volume (16) and the second volume (18), to pump the sample fluid (50) through the substrate (10), thereby allowing analyte to bind to the substrate. By opening the valve (24) and establishing a reversed pressure difference, sample fluid (50) bypasses the substrate (10) through the return channel (22). After closing the valve, the device is ready for a subsequent cycle, if desired. The device and method allow the analysis of in principle any amount of sample fluid, and the use of any type of substrate with respect to bubble pressure, and is thus more versatile and robust.

Claims21

Dependent claims

An analysis device (1;

100) for analyzing a sample fluid (50;
136) for the presence, absence or amount of an analyte in the sample fluid (50), the analysis device comprising
a substrate (10;
102) having a first surface (12) and an opposite second surface (14), and having a plurality of through going channels from the first surface (12) to the second surface (14), and at least partially being provided with a binding substance specific for the analyte,
a first volume (16;
106;
18;
108) in fluid communication with the first surface (12),
a second volume (18;
108;
16;
106) in fluid communication with the second surface (14), and
a pump (20, 60;
20′) for establishing a pressure difference between the first volume (16;
106;
18;
108) and the second volume (18;
108;
16;
106), the pump (20, 60;
20′) being operatively connected to the first volume (16;
106;
18;
108), characterized in that the device further comprises
a separate return channel (22;
124), for connecting the first volume (16;
106;
18;
108) and the second volume (18;
108;
16;
106) and thus allowing flow of the sample fluid (50;
136) from one of the first volume (16;
106;
18;
108) and the second volume (18;
108;
16;
106) to another of the first volume (16;
106;
18;
108) and the second volume (18;
108;
16;
106), and
a return channel valve (24;
126) that is able to close off the return channel (22;
124).

45 more elements

Dependencies are inferred from the claim text.

Facts

Application: 11/993,508Filed 23 Jun 2006
Priority: EP 05105884.030 Jun 2005
Published: 3 Jun 2010
Term ends: Not estimatedThe source records don’t support an estimate.

Classification4 codes

B01L 3/50857
For multiple samples, e.g. microtitration plates: using arrays or bundles of open capillaries for holding samples
B01L 3/5023
With fluid transport, e.g. in multi-compartment structures: with a sample being transported to, and subsequently stored in an absorbent for analysis
B01L 2400/0475
Moving fluids with specific forces or mechanical means: specific mechanical means and fluid pressure
B01L
Chemical or physical laboratory apparatus for general use