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US 2010/0136520 A1Published application
Detecting hepatitis b virus
AssigneeAbbott Laboratories
InventorsPaul F. Coleman, Akhtar X. Ali, Tanseem Ali
Life of this patent
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Abstract
This invention is related to methods for detecting hepatitis B virus by determining the level of hepatitis B virus surface antigen protein. This invention is also related to methods for detecting mutant hepatitis B virus surface antigen protein and kits for detecting hepatitis B virus.
Claims17
A method for detecting hepatitis B in a test sample, comprising:
- (a) providing a test sample;
- and
- (b) contacting said test sample with a first antibody for a time and under conditions sufficient to form middle hepatitis B surface protein (M-HBsAg)/first antibody complexes, presence of said complexes indicating detection of hepatitis B present said sample.
A method for detecting a mutant S-HBsAg in a test sample comprising:
- (a) providing a test sample;
- (b) contacting said test sample with a first antibody for a time and under conditions sufficient to form middle hepatitis B surface protein (M-HBsAg)/first antibody complexes;
- (c) contacting said test sample with a second antibody for a time and under conditions sufficient to form small hepatitis B surface protein (S-HBsAg)/second antibody complexes;
- and
- (d) comparing level of M-HBsAg/first antibody complexes to level of S-HBsAg/second antibody complexes, wherein a reduced level of S-HBsAg/second antibody complexes to M-HBsAg/first antibody complexes indicates detection of a mutant S-HBsAg in the test sample.
2 more elements
A method for detecting a mutant S-HBsAg in a test sample, comprising:
- (a) providing a test sample;
- (b) contacting said test sample with a first antibody for a time and under conditions sufficient to form small hepatitis B surface protein (S-HBsAg)/antibody complex
- wherein a reduced level of S-HBsAg/antibody complex in comparison to a control indicates detection of a mutant S-HBsAg in the test sample.
A kit for detecting hepatitis B comprising:
- (a) at least one antibody that binds to M-HBsAg;
- and
- (b) at least one antibody that binds to S-HBsAg.
Dependencies are inferred from the claim text.