Compositions for use in identification of papillomavirus
AssigneeIbis Biosciences, Inc.
InventorsRangarajan Sampath, Lawrence B. Blyn, Rachael Kreft, Thomas A. Hall, Feng Li
Life of this patent
Estimating the term…
Abstract
The present invention relates generally to identification of HPV, and provides methods, compositions and kits useful for this purpose when combined, for example, with molecular mass or base composition analysis.
Claims53
A composition comprising at least one purified oligonucleotide primer pair that comprises forward and reverse primers, wherein said primer pair comprises nucleic acid sequences that are substantially complementary to nucleic acid sequences of two or more different bioagents belonging to the HPV family, wherein said primer pair is configured to produce amplicons comprising different base compositions that correspond to said two or more different bioagents.
A composition comprising an isolated primer 15-35 bases in length selected from the group consisting of SEQ ID NOs 1-16.
A kit, comprising at least one purified oligonucleotide primer pair that comprises forward and reverse primers that are about 20 to 35 nucleobases in length, and wherein said forward primer comprises at least 70% sequence identity with a sequence selected from the group consisting of SEQ ID NOS:
- 1-8, and said reverse primer comprises at least 70% sequence identity with a sequence selected from the group consisting of SEQ ID NOS:
- 9-16.
A method of determining the presence of a HPV in at least one sample, the method comprising:
- (a) amplifying one or more segments of at least one nucleic acid from said sample using at least one purified oligonucleotide primer pair that comprises forward and reverse primers that are about 20 to 35 nucleobases in length, and wherein said forward primer comprises at least 70% sequence identity with a sequence selected from the group consisting of SEQ ID NOs:
- 1-8, and said reverse primer comprises at least 70% sequence identity with a sequence selected from the group consisting of SEQ ID NOs:
- 9-16 to produce at least one amplification product;
- and
- (b) detecting said amplification product, thereby determining said presence of said HPV in said sample.
A method of identifying one or more HPV bioagents in a sample, the method comprising:
- (a) amplifying two or more segments of a nucleic acid from said one or more HPV bioagents in said sample with two or more oligonucleotide primer pairs to obtain two or more amplification products;
- (b) determining two or more molecular masses and/or base compositions of said two or more amplification products;
- and
- (c) comparing said two or more molecular masses and/or said base compositions of said two or more amplification products with known molecular masses and/or known base compositions of amplification products of known HPV bioagents produced with said two or more primer pairs to identify said one or more HPV bioagents in said sample.
A system, comprising:
- (a) a mass spectrometer configured to detect one or more molecular masses of amplicons produced using at least one purified oligonucleotide primer pair that comprises forward and reverse primers, wherein said primer pair comprises nucleic acid sequences that are substantially complementary to nucleic acid sequences of two or more different HPV bioagents;
- and
- (b) a controller operably connected to said mass spectrometer, said controller configured to correlate said molecular masses of said amplicons with one or more HPV bioagent identities.
A purified oligonucleotide primer pair, comprising a forward primer and a reverse primer that each independently comprise 14 to 40 consecutive nucleobases selected from the primer pair sequences shown in Table 1 and/or Table 2, which primer pair is configured to generate an amplicon between about 50 and 150 consecutive nucleobases in length.
Dependencies are inferred from the claim text.