Assay for sars coronavirus by amplification and detection of nucleocapsid rna sequence
OwnerJianrong Lou, James A. Price, JR. +4inventors
InventorsJianrong Lou, James A. Price, JR., Daretta A. Yursis, David M. Wolfe, Lisa M. Keller, Tobin J. Hellyer
Life of this patent
Estimating the term…
Abstract
Primers and probes derived from SARS-CoV nucleic acid that facilitate detection and/or quantification of the nucleocapsid gene are disclosed. The disclosed sequences may be used in a variety of amplification and non-amplification formats for detection of SARSCoV infection.
Claims45
A oligonucleotide set comprising a first amplification primer and a second amplification primer, the first amplification primer consisting essentially of SEQ ID NO.:
- 4 or 18 and the second amplification primer consisting essentially of SEQ ID NO.:
- 5 or 19.
(canceled)
A oligonucleotide set comprising a first amplification primer and a second amplification primer, the first amplification primer consisting essentially of the target binding sequence of SEQ ID NO.:
- 4 or 18 and the second amplification primer consisting essentially of the target binding sequence of SEQ ID NO.:
- 5 or 19.
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
An oligonucleotide comprising a SARS Coronavirus (SARS-CoV) target sequence consisting essentially of SEQ ID NO.:
- 9, 10, 22 or 23.
A method for detecting the presence or absence SARS-CoV in a sample, the method comprising:
- (a) treating the sample with a plurality of nucleic acid primers in a nucleic acid amplification reaction wherein a first primer consists essentially of the target binding sequence of SEQ ID NO.:
- 4 or 18 and a second primer consists essentially of the target binding sequence of SEQ ID NO.:
- 5 or 19;
- and
- (b) detecting any amplified nucleic acid product, wherein detection of the amplified product indicates presence of SARS CoV.
39-45. (canceled)
A method for amplifying a target nucleic acid sequence of SARS-CoV comprising:
- (a) hybridizing to the nucleic acid
- (i) a first amplification primer consisting essentially of the target binding sequence of SEQ ID NO.:
- 4 or 18;
- and
- (ii) a second amplification primer consisting essentially of the target binding sequence of SEQ ID NO.:
- 5 or 19;
- and
- (b) extending the hybridized first and second amplification primers on the target nucleic acid sequence whereby the target nucleic acid sequence is amplified.
47-57. (canceled)
A method of quantifying the amount of SARS-CoV nucleic acid in a target sample comprising the steps of:
- a) combining the target sample with a known concentration of SARS-CoV internal control nucleic acid;
- b) amplifying the target nucleic acid and internal control nucleic acid in an amplification reaction;
- c) detecting the amplified nucleic acid;
- and
- d) analyzing the relative amounts of amplified SARS-CoV target nucleic acid and internal control nucleic acid.
61-63. (canceled)
Dependencies are inferred from the claim text.