Nanoemulsion and nanoparticle containing plant essential oil and method of production thereof

A method of production of a single layer nano emulsion comprising the steps of:

• adding plant essential oil of 0.03 to 0.3 wt % and sorbitan fatty acid ester of 0.09 to 1.5% to water soluble biopolymer of 0.05 to 1.0 wt %;
• adding water of the balance;
• and
• making the mixture into a nano emulsion.

A method of production of a double layer nano emulsion comprising the steps of:

• adding plant essential oil of 0.03 to 0.3 wt % and sorbitan fatty acid ester of 0.09 to 1.5% to one water soluble biopolymer of 0.05 to 1.0 wt %;
• adding water of the balance;
• adding calcium chloride to the mixture;
• making the mixture to which the calcium chloride is added into a nano emulsion;
• adding another water soluble biopolymer of 0.05 to 1.0 wt % to the nano emulsion and then, making it into a nano emulsion;
• and
• separating emulsions with nano size by centrifuging the nano emulsion.

A method of production of a triple layer nano emulsion comprising the steps of:

• adding plant essential oil of 0.03 to 0.3 wt % and sorbitan fatty acid ester of 0.09 to 1.5% to one water soluble biopolymer of 0.05 to 1.0 wt %;
• adding water of the balance;
• adding calcium chloride to the mixture;
• making the mixture to which the calcium chloride is added into a nano emulsion;
• adding another water soluble biopolymer of 0.05 to 1.0 wt % to the nano emulsion and then, making it into a nano emulsion;
• adding yet another water soluble biopolymer of 0.05 to 1.0 wt % to the nano emulsion and then, making it into a nano emulsion;
• and
• separating emulsions with nano size by centrifuging the nano emulsion.