Novel asparaginases and uses thereof
AssigneeDsm IP Assets B.V.
InventorsJan Metske Van Der Laan, Mark Cristiaan Stor, Ilse Lange De, Lisette Mohrmann
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Abstract
The present invention relates to an asparaginase having the width of the pH activity profile which is at least 3.5. Furthermore the invention relates to newly identified asparaginase polypeptide according to any one of SEQ ID NO: 2 or SEQ ID NO: 4 and to variants thereof and to polynucleotide sequences that encode such novel asparaginase variants. Furthermore the invention relates to the use of these novel asparaginase variants in industrial processes.
Claims20
An asparaginase which is:
- a) a polypeptide having an amino sequence as shown in any one of SEQ ID NO:
- 2 or SEQ ID NO:
- 4;
- b) a polypeptide which has at least 85% homology with a polypeptide according to a);
- c) a polypeptide which can be obtained from a polypeptide defined in a) or b) by substitution, deletion, and/or insertion of one or more amino acids;
- or
- d) a polypeptide which is encoded by a nucleic acid sequence according to any one of SEQ ID NO:
- 1 or SEQ ID NO:
- 3 encoding the mature polypeptide or a subsequence thereof having at least 100 nucleotides.
An asparaginase having the width of the pH activity profile which is at least 3.5, preferably at least 4, more preferably at least 5 pH units wide.
A nucleic acid sequence which comprises:
- a) a DNA sequence encoding the asparaginase according to any one of SEQ ID NO:
- 2 or SEQ ID NO:
- 4;
- b) a DNA sequence encoding an asparaginase which has at least 85% homology with the polypeptide according to any one of SEQ ID NO:
- 2 or SEQ ID NO:
- 4;
- c) a DNA sequence which has at least 80% homology with a DNA sequence according to a) or b);
- d) a DNA sequence which hybridizes at high stringency with a complementary strand of a DNA sequence according to a) or b);
- e) a subsequence of a DNA sequence according to a), b), c) or d) having at least 100 nucleotides;
- or
- f) a complementary strand of a DNA sequence according to a), b), c), d) or e),
Dependencies are inferred from the claim text.