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AssigneeAviex Technologies LLC
InventorDavid Gordon Bermudes
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The present invention relates to gram-negative bacterial mutants resistant to one or more stress conditions, including, but not limited to, CO2, acid pH, and high osmolarity. The present invention also relates more particularly to gram-negative bacterial mutants with reduced TNF-α induction having a mutation in one or more lipid biosynthesis genes, including, but not limited to msbB, that are rendered stress-resistant by a mutation in the zwf gene. The present invention provides compositions comprising one or more stress-resistant gram-negative bacterial mutants, preferably attenuated stress-resistant gram-negative bacterial mutants. In particular, the present invention relates to methods for prophylaxis or treatment of a virally induced disease in a subject comprising administering to said subject one or more stress-resistant gram-negative bacterial mutants, preferably attenuated stress-resistant gram-negative bacterial mutants. The present invention further relates to methods for prophylaxis or treatment of a virally induced disease in a subject comprising administering to said subject one or more stress-resistant gram-negative bacterial mutants as vectors for the delivery of one or more therapeutic molecules. The methods of the invention provide more efficient delivery of therapeutic molecules by stress-resistant gram-negative bacterial mutants engineered to express said therapeutic molecules.
A mutant Salmonella sp. comprising a genetically modified msbB gene and a mutation characterized by an increased growth when grown under at least one of high CO2 conditions, low pH media, and high osmolarity, compared to the msbB- mutant Salmonella designated YS1646.
A method for limiting the growth of a bacterial strain comprising the addition of gluconate to a media for the bacterial strain.
A live Salmonella bacteria, that is genetically engineered to express one or more viral antigens capable of viral prophylaxis or treatment having a first attenuating mutation that reduces TNF-α induction but confers sensitivity to CO2, osmolarity and/or acidic pH and a second mutation that confers resistance to CO2, osmolarity and/or acidic pH that maintains reduced TNF-α induction.
Dependencies are inferred from the claim text.