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US 2010/0135970 A1Published application
Methods for Reprogramming Adult Somatic Cells and Uses Thereof
AssigneeCaritas St. Elizabeth Medical Center of Boston, In
InventorsRaj Kishore, Douglas W. Losordo
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Abstract
As described below, the present invention features methods for reprogramming somatic cells and related therapeutic compositions and methods.
Claims28
A method for generating a reprogrammed cell, the method comprising:
- (a) contacting a somatic cell comprising a permeable cell membrane with an embryonic stem cell extract, thereby generating a de-differentiated cell;
- and
- (b) culturing the de-differentiated cell in the presence of at least one agent that induces differentiation, thereby generating a reprogrammed cell.
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A method of ameliorating an ischemic condition in a subject, the method comprising
- (a) contacting a fibroblast cell comprising a permeable cell membrane with an embryonic stem cell extract in an ATP regenerating buffer;
- (b) culturing the cell in the presence of LIF and BMP-2 to generate an endothelial cell;
- and
- (c) administering the endothelial cell of step (b) into a muscle tissue of the subject, thereby ameliorating an ischemic condition.
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Dependencies are inferred from the claim text.