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US 2010/0133430 A1Published application
Ms methods to evaluate glycans
AssigneeMomenta Pharmaceuticals, Inc.
InventorsCarlos J. Bosques, Nathaniel J. Washburn, Xiangping Zhu, Ian Christopher Parsons
Life of this patent
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Abstract
The present disclosure provides, among other things, methods for the identification of sulfated glycans in a mixture of glycans.
Claims23
A method for identifying sulfated glycans in a mixture of glycans, comprising the steps of:
- providing a mass spectrum for a mixture of glycans;
- determining for multiple pairs of mass peaks in the mass spectrum separated by two mass units, [M] and [M+2], the signal strength ratio [M+2]/[M] for each pair;
- and identifying a mass peak associated with a first glycan as that of a sulfated glycan when at least the [M+2]/[M] ratio associated with the first glycan is greater than the [M+2]/[M] ratio associated with mass peaks corresponding to one or more similar glycans by a selected threshold.
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A method for identifying sulfated glycans in a mixture of glycans, comprising the steps of:
- providing a mass spectrum for a mixture of glycans;
- determining for multiple pairs of mass peaks in the mass spectrum separated by two mass units, [M] and [M+2], the signal strength ratio [M+2]/[M] for each pair;
- and identifying a mass peak as arising from a sulfated glycan based at least on the distribution of [M+2]/[M] ratios for three or more similar glycans.
-22. (canceled)
A method for identifying sulfated glycans in a mixture of glycans, comprising the steps of:
- providing a mass spectrum for a mixture of glycans;
- determining for at least one pair of mass peaks in the mass spectrum separated by two mass units, [M] and [M+2], the signal strength ratio [M+2]/[M];
- and identifying a mass peak associated with the glycan as that of a sulfated glycan when at least the [M+2]/[M] associated with the glycan is similar to the [M+2]/[M] of a computer-generated mass spectra for a theoretical sulfated glycan.
(canceled)
Dependencies are inferred from the claim text.