Method for evaluating target molecules

A method for evaluating a target molecule bound to a probe molecule provided with a marker, comprising:

• applying AC voltage between a working electrode provided on a substrate and a counter electrode;
• and
• using an amplitude strength of a signal obtained from the marker on the probe molecule bound to the working electrode when a frequency of the AC voltage is varied to determine a Stokes radius or molecular weight of the target molecule.

A method for evaluating a target molecule bound to a probe molecule provided with a marker, comprising:

• applying AC voltage between a working electrode provided on a substrate and a counter electrode;
• and
• using an average value of a signal obtained from the marker on the probe molecule bound to the working electrode when a frequency of the AC voltage is varied to determine a Stokes radius or molecular weight of the target molecule.

A method for evaluating a target molecule bound to a probe molecule provided with a marker, comprising:

• applying AC voltage between a working electrode provided on a substrate and a counter electrode;
• and
• using an average value of a signal obtained from the marker on the probe molecule bound to the working electrode when a frequency of the AC voltage is varied to determine at least one selected from a binding rate between the probe molecule and the target molecule, a binding rate constant therebetween, a dissociation rate between the probe molecule and the target molecule, and a dissociation rate constant therebetween.