Bifidobacterial gene sequences and their use
AssigneeThe Regents of the University of California
InventorsDavid A. Mills, Carlito B. Lebrilla, J. Bruce German, David Sela
Life of this patent
Estimating the term…
Abstract
This invention provides nucleic acids and proteins involved in oligosaccharide modification in the species Bifidobacteria. The invention provides methods for utilizing the proteins of the invention to generate human milk oligosaccharides or oligosaccharide mimics. The invention also provides compositions containing the human milk oligosaccharides or oligosaccharide mimics and methods for use.
Claims31
An isolated nucleic acid encoding a polypeptide involved in oligosaccharide modification that hybridizes under high stringency conditions to a sequence selected from the group consisting of:
- SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, and 22.
A polypeptide involved in oligosaccharide modification of amino acid sequence at least 90% identical to the amino acid sequence encoded by a nucleic acid selected from the group consisting of SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, and 22.
A method of synthesizing human milk oligosaccharides or oligosaccharide mimics, the method comprising the steps of:
- (a) contacting an oligosaccharide containing precursor with a polypeptide with an amino acid sequence at least 90% identical to the amino acid sequence encoded by a nucleic acid selected from the group consisting of SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, and 22;
- and
- (b) isolating a modified oligosaccharide containing precursor.
A human milk oligosaccharide or oligosaccharide mimic derived by a method comprising the steps of (a) contacting an oligosaccharide containing precursor with a polypeptide of amino acid sequence at least 90% identical to the amino acid sequence encoded by a nucleic acid selected from the group consisting of SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, and 22;
- and (b) isolating the resultant modified oligosaccharide precursor.
A milk product enriched for human milk oligosaccharides or oligosaccharide mimics derived by a method comprising the steps of contacting a starting milk product with a polypeptide of amino acid sequence at least 90% identical to the amino acid sequence encoded by a nucleic acid selected from the group consisting of SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, and 22.
Dependencies are inferred from the claim text.