Two-Step Enzyme Method for Preparing 7-Aminocephalosporanic Acid
AssigneeBioright Worldwide Company Limited
InventorsJun Wang, Waikei Tsang, Hongkin Yap, Junmin Chen, Yaulung Siu, Supyin Tsang
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Abstract
The present invention discloses a two-step enzyme method for preparing 7-aminocephalosporanic acid from cephalosporin C, wherein D-amino acid oxidase used is purified D-amino acid oxidase mutant of yeast Trigonopsis variabilis, having a specific activity of 105% higher than that of parent D-amino acid oxidase. The method has no need of addition of hydrogen peroxide, β-lactamase inhibitor, catalase inhibitor, catalase and the like commonly used in the prior art. The productivity of the method can reach more than 93%. Thus, the method is simple, low in cost and high in productivity.
Claims11
1-10. (canceled)
A method to prepare 7-ACA from CPC, which comprises the conversion of CPC to glutaryl-7-ACA by D-amino acid oxidase (first step reaction) and the conversion of glutaryl-7-ACA to 7-ACA by glutaryl-7-ACA acylase (second step reaction), wherein said D-amino acid oxidase is a purified Trigonopsis variabilis D-amino acid oxidase mutant with the amino acid sequence as in Sequence 2.
Dependencies are inferred from the claim text.