Methods and Compositions for Increasing Tissue Tropism of Recombinant Adenoviral Vectors
AssigneesCommonwealth Scientific and Industrial Research Organisation, Imugene Limited
InventorsJeffrey Hammond, Michael A. Johnson
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Abstract
Methods are provide to allow for the preparation of adenoviral vectors with altered tropism. Compositions comprising such vectors and methods of use thereof also are provided.
Claims29
A recombinant adenoviral vector comprising an adenovirus that comprises a fibre gene native to said adenovirus and further comprises a second fibre gene that is heterologous to said adenovirus, wherein said second fibre gene is acquired by said recombinant adenovirus by growth of said recombinant adenovirus in a cell line that stably expresses said second fibre gene.
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A host cell comprising an adenovirus that comprises a fibre gene native to said porcine adenovirus and wherein said host cell is a recombinant cell that expresses a fibre gene that is heterologous to said adenovirus and is capable of being infected by porcine adenovirus.
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A method of preparing an adenovirus comprising:
- a. culturing a recombinant host cell that expresses an adenoviral fibre gene under conditions suitable for infection of said cell with adenovirus, b. contacting said cell with a recombinant adenovirus vector which comprises the adenovirus sequence(s) essential for encapsidation and a heterologous gene that encodes a heterologous protein and wherein said recombinant adenovirus comprises a fibre gene that is different from the fibre gene in said host cell;
- and optionally harvesting said adenovirus.
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A composition comprising a host cell that expresses an adenovirus fibre gene and a recombinant adenoviral vector that comprises nucleic acid that encodes a heterologous protein under the control of an expression control sequence, wherein said recombinant adenoviral vector comprises a fibre gene that is native to the adenovirus of said vector.
A method of increasing the host tissue cell specificity of a recombinant adenovirus vector comprising growing said recombinant adenovirus in a host cell that comprises a second fibre protein that is different from the fibre protein of said recombinant adenovirus.
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Dependencies are inferred from the claim text.