Retentate chromatography and protein chip arrays with applications in biology and medicine
AssigneeBio-Rad Laboratories, Inc.
InventorsT. William Hutchens, Tai-Tung Yip
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Abstract
This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.
Claims21
1-8. (canceled)
A method for determining whether an analyte is differentially present in a first and second biological sample comprising the steps of:
- a) determining a first retention map for the analyte in the first sample for at least one selectivity condition;
- b) determining a second retention map for the analyte in the second sample for the same selectivity condition;
- and
- c) detecting a difference between the first and the second retention maps;
- whereby a difference in the retention maps provides a determination that the analyte is differentially present in first and second samples.
A method of diagnosing in a subject a disease characterized by at least one diagnostic marker comprising the steps of:
- a) providing a substrate for use in desorption spectrometry that comprises at least one addressable location, each addressable location comprising an adsorbent that resolves at least one of the diagnostic markers under an elation condition;
- b) exposing the substrate to a biological sample from the subject under the elution condition to allow retention of the diagnostic marker;
- and
- c) detecting retained diagnostic marker by desorption spectrometry;
- whereby detecting retained diagnostic marker provides a diagnosis of the disease.
A kit for detecting an analyte in a sample comprising (1) a substrate for use in desorption spectrometry that comprises at least one addressable location, each addressable location comprising an adsorbent that resolves an analyte under a selectivity condition comprising the adsorbent and an eluant, and (2) the eluant or instructions for exposing the sample to the selectivity condition.
28-30. (canceled)
Dependencies are inferred from the claim text.